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首页> 美国卫生研究院文献>Molecules >Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property
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Enhanced Production of Gypenoside LXXV Using a Novel Ginsenoside-Transforming β-Glucosidase from Ginseng-Cultivating Soil Bacteria and Its Anti-Cancer Property

机译：人参栽培土壤细菌中一种新型人参皂苷转化β-葡萄糖苷酶提高人参皂苷LXXV的产量及其抗癌性能

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摘要

Minor ginsenosides, such as compound K, Rg3(S), which can be produced by deglycosylation of ginsenosides Rb1, showed strong anti-cancer effects. However, the anticancer effects of gypenoside LXXV, which is one of the deglycosylated shapes of ginsenoside Rb1, is still unknown due to the rarity of its content in plants. Here, we cloned and characterized a novel ginsenoside-transforming β-glucosidase (BglG167b) derived from Microbacterium sp. Gsoil 167 which can efficiently hydrolyze gypenoside XVII into gypenoside LXXV, and applied it to the production of gypenoside LXXV at the gram-scale with high specificity. In addition, the anti-cancer activity of gypenoside LXXV was investigated against three cancer cell lines (HeLa, B16, and MDA-MB231) in vitro. Gypenoside LXXV significantly reduced cell viability, displaying an enhanced anti-cancer effect compared to gypenoside XVII and Rb1. Taken together, this enzymatic method would be useful in the preparation of gypenoside LXXV for the functional food and pharmaceutical industries.
机译：可以通过人参皂苷Rb1的去糖基化作用生成的次要人参皂苷（例如化合物K，Rg3（S））具有很强的抗癌作用。但是，人参皂甙Rb1的去糖基化形式之一的人参皂甙LXXV的抗癌作用由于在植物中的含量很少而仍然未知。在这里，我们克隆和表征了一种新的人参皂苷转化β-葡萄糖苷酶（BglG167b），来源于微细菌属。可以有效地将人参皂苷XVII水解为人参皂苷LXXV的Gsoil 167，以高克数的克级应用在人参皂苷LXXV的生产中。此外，在体外研究了绞股蓝皂苷LXXV对三种癌细胞系（HeLa，B16和MDA-MB231）的抗癌活性。与绞股蓝皂苷XVII和Rb1相比，绞股蓝皂苷LXXV显着降低了细胞活力，显示出增强的抗癌作用。两者合计，这种酶促方法将在功能性食品和制药工业的绞股蓝皂苷LXXV的制备中有用。

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