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首页> 美国卫生研究院文献>Molecules >Development of a Rapid and Simple Method for Preparing Tea-Leaf Saponins and Investigation on Their Surface Tension Differences Compared with Tea-Seed Saponins
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Development of a Rapid and Simple Method for Preparing Tea-Leaf Saponins and Investigation on Their Surface Tension Differences Compared with Tea-Seed Saponins

机译：一种快速简便的制备茶叶皂苷的方法及其与茶籽皂苷相比表面张力差异的研究

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摘要

The relative overcapacity in China's tea-leaf production and the potential application of tea-leaf saponins in soil remediation encouraged in-depth developments and comprehensive utilizations of tea-leaf resources. Through variables optimizations using Box–Behnken designs for ultrasonic power, temperature as well as ultrasonic treatment time in ultrasonic-assisted water extraction and single-variable experiments for acetone-extraction solution ratio in acetone precipitation, a rapid and simple method was developed for preparing tea-leaf saponins. Tea-leaf saponins with the concentration of 3.832 ± 0.055 mg/mL and the purity of 76.5% ± 1.13% were acquired under the optimal values of 78 w, 60 °C, 20 min and 0.1 ratio of acetone-extraction solution. Both Fourier transform-infrared (FT-IR) spectra and ultraviolet (UV) spectra revealed slight composition differences between tea-leaf saponins and tea-seed saponins, while these differences were not reflected in the critical micelle concentration (CMC) and the surface tension of tea-leaf saponins and tea-seed saponins, indicating there was no need to distinguish them at the CMC. Further research attention on where tea-leaf saponins were in low concentrations is deserved to discover whether they had differences in comparison with tea-seed saponins, which was beneficial to apply them in the phytoremediation of contaminated soils.
机译：中国茶叶生产能力相对过剩，以及茶叶皂苷在土壤修复中的潜在应用，促进了茶叶资源的深入开发和综合利用。通过使用Box–Behnken设计对超声波辅助水提取中的超声功率，温度和超声处理时间进行变量优化，以及对丙酮沉淀中丙酮提取液比率的单变量实验，开发了一种快速简便的茶制备方法-叶皂苷。在78 w，60°C，20 min和0.1比例的丙酮萃取溶液的最佳条件下，获得了3.832±0.055 mg / mL浓度的茶叶皂素，纯度为76.5％±1.13％。傅立叶变换红外（FT-IR）光谱和紫外（UV）光谱均显示茶叶皂角苷和茶籽皂苷之间的成分差异很小，而这些差异并未反映在临界胶束浓度（CMC）和表面张力中茶叶皂苷和茶籽皂苷的含量，这表明不需要在CMC上进行区分。值得进一步研究的重点是茶叶皂素的低浓度，以发现它们与茶籽皂苷相比是否有差异，这有利于将其应用于污染土壤的植物修复。

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