目的：建立超高效液相色谱法测定三七中三七皂苷R1、人参皂苷Rg1、人参皂苷Rb1含量的方法。方法采用Agilent 1290型高效液相色谱仪, ZORBAX Eclipse Plus C18(2.1×50 mm,1.8μm)色谱柱,以乙腈-水为流动相,梯度洗脱,流速为0.22 ml/min,检测波长为203 nm,进样量为0.4μl,柱温为40℃。结果三七中三七皂苷R1、人参皂苷Rg1和人参皂苷Rb1在25 min内实现完全分离,峰面积在选定的浓度范围内线性良好,方法回收率、精密度良好。结论超高效液相色谱法可以作为三七药材质量控制的一种快速、方便、可靠的检测手段。%Objective To establish the determination method for notoginsenoside R1 and ginsenoside Rg1 and ginsenoside Rb1 in panax notoginseng by ultra high performance liquid chromatography. Methods This study was performed on Agilent 1290 high performance liquid chromatography and ZORBAX Ecilpse Plus C18 (2.1×50 mm, 1.8μm) chromatographic column with acetonitrile-water as mobile phase in gradient elution at the flow rate of 0.220 ml/min. The detection wavelength was 203 nm, the injection volume was 0.4μl, and the column temperature was 40℃. Results Notoginsenoside R1, ginsenosides Rg1 and ginsenosides Rb1 in panax notoginseng could be completely separated in 25 min. The peak area had good linear relationship in the certain range. The recovery rate and precision of the method were good. Conclusion As an new quality control method of panax notoginseng, ultra high performance liquid chromatography is fast, convenient and reliable.
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