• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Molecules >Metabolism Study of Notoginsenoside R1 Ginsenoside Rg1 and Ginsenoside Rb1 of Radix Panax Notoginseng in Zebrafish
【2h】

Metabolism Study of Notoginsenoside R1 Ginsenoside Rg1 and Ginsenoside Rb1 of Radix Panax Notoginseng in Zebrafish

机译：人参中三七皂苷R1人参皂苷Rg1和人参皂苷Rb1在斑马鱼中的代谢研究

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Zebrafish, a common model organism for studies of vertebrate development and gene function, has been used in pharmaceutical research as a new and powerful tool in recent years. In the present study, we applied zebrafish for the first time in a metabolic study of notoginsenoside (R1), ginsenoside (Rg1) and ginsenoside (Rb1), which are saponins isolated from Panax notoginseng. Metabolites of these three saponin compounds in zebrafish after exposure for 24 h were identified by high performance liquid chromatography - electrospray mass spectrometry (HPLC-ESI-MS) with a Zorbax C-18 column for separation using a binary gradient elution of 0.05% formic acid acetonitrile - 0.05% formic acid water. The quasi-molecular ions of compounds were detected in negative mode. Step-wise deglycosylation metabolites and hydroxylation metabolites of the three saponins were found, which were coincide with regular methods for metabolic analysis. Our study demonstrated that the zebrafish model can successfully imitate the current metabolic model with advantages of lower cost, far less amount of compound needed, easy set up and high performance. Our data suggests that the zebrafish metabolic model has the potential for developing a novel method for quickly predicting the metabolism of Chinese herb components, including those of trace compounds.
机译：斑马鱼是研究脊椎动物发育和基因功能的常见模式生物，近年来已在药物研究中用作一种新的强大工具。在本研究中，我们在三七皂苷（R1），人参皂苷（Rg1）和人参皂苷（Rb1）的代谢研究中首次应用斑马鱼，它们是从三七提取的皂苷。通过高效液相色谱-电喷雾质谱（HPLC-ESI-MS）和Zorbax C-18色谱柱，使用0.05％甲酸的二元梯度洗脱进行分离，从而确定了斑马鱼暴露24 h后这三种皂苷化合物的代谢物。乙腈-0.05％甲酸水。以负模式检测化合物的准分子离子。发现了三个皂苷的逐步去糖基化代谢物和羟基化代谢物，这与常规的代谢分析方法是一致的。我们的研究表明，斑马鱼模型可以成功地模仿当前的代谢模型，具有成本低，所需化合物少得多，易于设置和高性能的优点。我们的数据表明，斑马鱼的代谢模型具有开发一种新方法的潜力，该方法可以快速预测中草药成分（包括微量化合物的成分）的代谢。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号