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首页> 外文会议>International Conference on Applied Sciences >Effect of enzyme on extraction of ginsenoside Rb1 and Rg3 from Panax notoginseng roots
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Effect of enzyme on extraction of ginsenoside Rb1 and Rg3 from Panax notoginseng roots

机译：酶对人参皂苷RB1和RG3提取的影响来自Panax Notoginseng根

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Panax notoginseng is distributed throughout the north and northwest of Vietnam, especially Ha Giang, Lao Cai, and Cao Bang provinces. The root of this plant contains ginsenosides (Rb1, Rb2, Rd, Rg3), flavonoids, polyacetylene, polysaccharides, amino acids, fatty acids, and peptides. In this study, the ratios of enzyme (Viscozyme, Termamyl, Cellulase), solvent of components, and time extraction were investigated. The results showed that the highest contents of Rb1 and Rg3 were achieved in the sample extracted with the ratio of enzymes V:C:T = 1:0:0, ethanol:water (60:40, v/v) as extracting solvent in 45 minutes. Then, conditions of high performance liquid chromatography with diode array detector method to determine the content of ginsenosides Rb1 and Rg3 in the roots of Panax notoginseng were studied, including wavelength, mobile phase, and flow rate. The separation was subjected on a reversed-phase C18 column using acetonitrile (A) and water (B) as mobile phase. The gradient elution was set as follow: 0-10 min, 15-25% A; 10-20 min, 25-30% A; 20-40 min, 30-60% A; 40-60 min, 60-80% A; and 60-65 min back to 15% A before the next injection, at a flow rate of 0.5 mL/min, and the wavelength was set at 202 nm. The linear range was from 298.59 to 696.72μg/mL for Rb1 and from 8.19 to 19.10μg/L for Rg3. The limits of detection for Rb1 and Rg3 obtained were 0.31μg/mL and 0.33μg/mL, respectively. The limits of quantification were 0.95μg/mL and 1.01μg/mL for Rb1 and Rg3, respectively. Consequently, the high performance liquid chromatography demonstrated the highly sensitive and accurate method for determination of Rb1 and Rg3 in Panax notoginseng.
机译：Panax Noginseng分布在越南的北部和西北部，特别是Ha Giang，Lao Cai和Cao Bang Provinces。该植物的根部含有人参皂苷（RB1，RB2，Rd，RG3），黄酮类，聚乙炔，多糖，氨基酸，脂肪酸和肽。在该研究中，研究了酶（Viscoozyme，末端，纤维素酶），组分溶剂和时间萃取的比率。结果表明，用酶V：C：T = 1：0：0，乙醇：水（60:40，v / v）萃取的样品中达到最高含量的RB1和RG3。作为提取溶剂45分钟。然后，研究了具有二极管阵列检测器方法的高效液相色谱的条件，以确定人参皂苷RB1和RG3中的人参皂苷RB1和RG3的含量，包括波长，流动相和流速。使用乙腈（A）和水（B）作为流动相，在反相C18柱上进行分离。梯度洗脱设定如下：0-10分钟，15-25％a; 10-20分钟，25-30％a; 20-40分钟，30-60％a; 40-60分钟，60-80％a;在下一次注射之前，60-65分钟回到15％A，流速为0.5ml / min，并且波长设定为202nm。线性范围为RB1的298.59至696.72μg/ ml，RG3的8.19至19.10μg/ L.得到的RB1和RG3的检测限为0.31μg/ ml和0.33μg/ ml。定量极限分别为0.95μg/ ml，RB1和RG3分别为1.01μg/ ml。因此，高性能液相色谱法证明了在Panax Notinseng中测定RB1和RG3的高敏感和准确的方法。

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