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首页> 美国卫生研究院文献>Molecules >Antifungal Antibacterial and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds
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Antifungal Antibacterial and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

机译：金合欢（Labill。）H.L.Wendl的抗真菌抗菌和抗氧化活性。花提取物：酚类和类黄酮化合物的HPLC分析

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In this study, for the environmental development, the antifungal, antibacterial, and antioxidant activities of a water extract of flowers from Acacia saligna (Labill.) H. L. Wendl. were evaluated. The extract concentrations were prepared by dissolving them in 10% DMSO. Wood samples of Melia azedarach were treated with water extract, and the antifungal activity was examined at concentrations of 0%, 1%, 2%, and 3% against three mold fungi; Fusarium culmorum , Rhizoctonia solani , and Penicillium chrysogenum that cause root rot, cankers, and green fruit rot, respectively, isolated from infected Citrus sinensis L. Antibacterial evaluation of the extract was assayed against four phytopathogenic bacteria, including Agrobacterium tumefaciens, Enterobacter cloacae, Erwinia amylovora, and Pectobacterium carotovorum subsp. carotovorum, using the micro-dilution method to determine the minimum inhibitory concentrations (MICs). Further, the antioxidant capacity of the water extract was measured via 2,2′-diphenylpicrylhydrazyl (DPPH). Phenolic and flavonoid compounds in the water extract were analyzed using HPLC: benzoic acid, caffeine, and o-coumaric acid were the most abundant phenolic compounds; while the flavonoid compounds naringenin, quercetin, and kaempferol were identified compared with the standard flavonoid compounds. The antioxidant activity of the water extract in terms of IC50 was considered weak (463.71 μg/mL) compared to the standard used, butylated hydroxytoluene (BHT) (6.26 μg/mL). The MIC values were 200, 300, 300, and 100 µg/mL against the growth of A. tumefaciens, E. cloacae, E. amylovora, and P. carotovorum subsp. carotovorum, respectively, which were lower than the positive control used (Tobramycin 10 μg/disc). By increasing the extract concentration, the percentage inhibition of fungal mycelial was significantly increased compared to the control treatment, especially against P. chrysogenum, suggesting that the use of A. saligna flower extract as an environmentally friendly wood bio-preservative inhibited the growth of molds that cause discoloration of wood and wood products.
机译：在这项研究中，对于环境发展，来自阿拉伯洋槐（Labill。）H. L. Wendl的花朵的水提取物的抗真菌，抗菌和抗氧化活性。被评估。通过将提取物浓度溶解在10％DMSO中来制备。用水提取物处理of木的木材样品，并以0％，1％，2％和3％的浓度对三种霉菌进行抗真菌活性测试；从感染的柑橘中分别分离出引起根腐烂，枯萎病和绿色水果腐烂的镰刀菌，茄形假单胞菌和产青青霉。对提取物的四种细菌致病菌（包括根癌土壤杆菌，阴沟肠杆菌，欧文氏杆菌）进行了抗菌评估。支链淀粉菌和胡萝卜杆菌芽孢亚种。用微稀释法确定最小抑菌浓度（MICs）。此外，水提取物的抗氧化能力是通过2，2'-二苯基吡啶并肼基（DPPH）测量的。使用HPLC分析了水提取物中的酚类和黄酮类化合物：苯甲酸，咖啡因和邻香豆酸是含量最高的酚类化合物。而黄酮类化合物柚皮苷，槲皮素和山奈酚的含量与标准黄酮类化合物相比得到了确认。与使用的标准丁基化羟基甲苯（BHT）（6.26μg/ mL）相比，水提取物的抗氧化活性（IC50）被认为很弱（463.71μg/ mL）。相对于根癌农杆菌，阴沟肠杆菌，支链淀粉肠杆菌和胡萝卜假单胞菌亚种的生长，MIC值为200、300、300和100 µg / mL。胡萝卜素分别低于使用的阳性对照（妥布霉素10μg/碟）。与对照相比，通过增加提取物的浓度，真菌菌丝体的抑制百分比显着增加，尤其是针对产黄青霉。对表明，使用五味子花提取物作为环保的木材生物防腐剂可以抑制霉菌的生长。导致木材和木制品变色。

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