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首页> 外文期刊>Molecules >Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds
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Antifungal, Antibacterial, and Antioxidant Activities of Acacia Saligna (Labill.) H. L. Wendl. Flower Extract: HPLC Analysis of Phenolic and Flavonoid Compounds

机译：抗真菌，抗菌和抗氧化剂活性的Acacia saligna（Labill.）H. L.Wendl。 花提取物：酚类和黄酮化合物的HPLC分析

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In this study, for the environmental development, the antifungal, antibacterial, and antioxidant activities of a water extract of flowers from Acacia saligna (Labill.) H. L. Wendl. were evaluated. The extract concentrations were prepared by dissolving them in 10% DMSO. Wood samples of Melia azedarach were treated with water extract, and the antifungal activity was examined at concentrations of 0%, 1%, 2%, and 3% against three mold fungi; Fusarium culmorum MH352452, Rhizoctonia solani MH352450, and Penicillium chrysogenum MH352451 that cause root rot, cankers, and green fruit rot, respectively, isolated from infected Citrus sinensis L. Antibacterial evaluation of the extract was assayed against four phytopathogenic bacteria, including Agrobacterium tumefaciens, Enterobacter cloacae, Erwinia amylovora, and Pectobacterium carotovorum subsp. carotovorum, using the micro-dilution method to determine the minimum inhibitory concentrations (MICs). Further, the antioxidant capacity of the water extract was measured via 2,2-diphenylpicrylhydrazyl (DPPH). Phenolic and flavonoid compounds in the water extract were analyzed using HPLC: benzoic acid, caffeine, and o-coumaric acid were the most abundant phenolic compounds; while the flavonoid compounds naringenin, quercetin, and kaempferol were identified compared with the standard flavonoid compounds. The antioxidant activity of the water extract in terms of IC50 was considered weak (463.71 g/mL) compared to the standard used, butylated hydroxytoluene (BHT) (6.26 g/mL). The MIC values were 200, 300, 300, and 100 mu g/mL against the growth of A. tumefaciens, E. cloacae, E. amylovora, and P. carotovorum subsp. carotovorum, respectively, which were lower than the positive control used (Tobramycin 10 g/disc). By increasing the extract concentration, the percentage inhibition of fungal mycelial was significantly increased compared to the control treatment, especially against P. chrysogenum, suggesting that the use of A. saligna flower extract as an environmentally friendly wood bio-preservative inhibited the growth of molds that cause discoloration of wood and wood products.
机译：在本研究中，对于环境开发，抗真菌，抗菌和抗氧化和抗氧化和抗氧化活性从Acacia Saligna（Labill.）H. L.Wendl。评估了。通过将它们溶解在10％DMSO中来制备提取物浓度。用水提取物处理Melia Azedarach的木样品，并以0％，1％，2％和3％的浓度检查抗真菌活性，抵抗三种模具真菌; Fusarium culmorum mh352452，rhizoctonia solani mh352450和penicillium chrysogenum mh352451分别导致从感染的柑橘sinensis l的根腐肉，溃疡和绿色水果腐蚀。针对四种植物疗法细菌测定提取物的抗菌评估，包括肿瘤术，肠球Cloacae，Erwinia Amylovora和CeCtobacterium carotovorum subsp。 Carotovorum，使用微量稀释方法来确定最小抑制浓度（MIC）。此外，通过2,2-二苯基吡啶基酰基（DPPH）测量水提取物的抗氧化能力。使用HPLC：苯甲酸，咖啡因和O-香豆酸分析水提取物中的酚类和黄酮化合物是最丰富的酚类化合物;与标准的类黄酮化合物相比，鉴定了类黄酮化合物Naringenin，槲皮素和Kaempferol。与所用的标准，丁基化羟基甲苯（BHT）（6.26g / ml）相比，水提取物在IC 50方面的抗氧化活性被认为是弱（463.71g / ml）。麦克风值为200,300,300和100μg/ ml，其生长为A. tumefaciens，E.Cloacae，E.Amylovora和P.Carotovorum Subsp。 Carotovorum分别低于所用阳性对照（致苄霉素10g /圆盘）。通过提高提取物浓度，与对照治疗相比，真菌菌丝体的抑制率显着增加，特别是针对甲醇糖原，表明使用A.苏尔语花提取物作为环保木生物防腐剂抑制了模具的生长导致木材和木制品变色。

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