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首页> 美国卫生研究院文献>Molecules >In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on Caenorhabditis elegans
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In Vivo and In Vitro Antioxidant Activities of Methanol Extracts from Olive Leaves on Caenorhabditis elegans

机译：橄榄叶甲醇提取物对秀丽隐杆线虫的体内和体外抗氧化活性

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The aim of this study was to evaluate the antioxidant activities of extracts from olive leaves (EOL). The main contents of EOL were determined by colorimetric methods. The antioxidant activities were assessed by measuring the scavenging free radicals in vitro. To investigate the antioxidant activity in vivo, we detected the survival of Caenorhabditis elegans, under thermal stress. Subsequently the reactive oxygen species (ROS) level, activities of antioxidant enzymes, the expression of HSP-16.2 and the translocation of daf-16 were measured. The results showed that, polyphenols was the main component. EOL could well scavenge DPPH and superoxide anion radicals in vitro. Compared to the control group, the survival rate of C. elegans treated with EOL was extended by 10.43%, under heat stress. The ROS level was reduced, while the expression of hsp-16.2 was increased to protect the organism against the increasing ROS. The level of malondialdehyde (MDA) also decreased sharply. The activities of inner antioxidant enzymes, such as catalase (CAT), superoxide dismutase (SOD), and glutathione peroxidase (GSH-PX) were potentiated, which might have had a correlation with the DAF-16 transcription factor that was induced-turned into the nuclear. Therefore, EOL showed a strong antioxidant ability in vitro and in vivo. Hence, it could be a potential candidate when it came to medicinal and edible plants.
机译：这项研究的目的是评估橄榄叶提取物（EOL）的抗氧化活性。 EOL的主要含量通过比色法测定。通过测量体外清除自由基来评估抗氧化活性。为了研究体内的抗氧化活性，我们检测了秀丽隐杆线虫在热应激下的存活。随后测量了活性氧（ROS）水平，抗氧化酶的活性，HSP-16.2的表达和daf-16的易位。结果表明，多酚是主要成分。 EOL可以很好地清除DPPH和超氧阴离子自由基。与对照组相比，用EOL处理的秀丽隐杆线虫在热胁迫下的存活率延长了10.43％。 ROS水平降低，而hsp-16.2的表达增加，以保护生物体免受ROS的增加。丙二醛（MDA）的含量也急剧下降。增强了内部抗氧化酶的活性，例如过氧化氢酶（CAT），超氧化物歧化酶（SOD）和谷胱甘肽过氧化物酶（GSH-PX），这可能与被诱导转化为DAF-16转录因子的相关性核。因此，EOL在体外和体内均显示出强大的抗氧化能力。因此，在药用和食用植物方面，它可能是潜在的候选者。

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