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首页> 美国卫生研究院文献>Molecules >Comparison between Exhaustive and Equilibrium Extraction Using Different SPE Sorbents and Sol-Gel Carbowax 20M Coated FPSE Media
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Comparison between Exhaustive and Equilibrium Extraction Using Different SPE Sorbents and Sol-Gel Carbowax 20M Coated FPSE Media

机译：使用不同的SPE吸附剂和Sol-Gel Carbowax 20M涂层FPSE介质进行穷举和平衡萃取的比较

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摘要

This paper reports the performance comparison between the exhaustive and equilibrium extraction using classical Avantor C18 solid phase extraction (SPE) sorbent, hydrophilic-lipophilic balance (HLB) SPE sorbent, Sep-Pak C18 SPE sorbent, novel sol-gel Carbowax 20M (sol-gel CW 20M) SPE sorbent, and sol-gel CW 20M coated fabric phase sorptive extraction (FPSE) media for the simultaneous extraction and analysis of three inflammatory bowel disease (IBD) drugs that possess logP values (polarity) ranging from 1.66 for cortisone, 2.30 for ciprofloxacin, and 2.92 for sulfasalazine. Both the commercial SPE phases and in-house synthesized sol-gel CW 20M SPE phases were loaded in SPE cartridges and the extractions were carried out under an exhaustive extraction mode. FPSE was carried out under an equilibrium extraction mode. The drug compounds were resolved using a Luna C18 column (250 mm × 4.6 mm; 5 μm particle size) in gradient elution mode within 20 min and the method was validated in compliance with international guidelines for the bioanalytical method validation. Novel in-house synthesized and loaded sol-gel CW 20M SPE sorbent cartridges were characterized in terms of their extraction capability, breakthrough volume, retention volume, hold-up volume, number of the theoretical plate, and the retention factor.
机译：本文报告了使用经典Avantor C18固相萃取（SPE）吸附剂，亲水-亲脂平衡（HLB）SPE吸附剂，Sep-Pak C18 SPE吸附剂，新型溶胶-凝胶Carbowax 20M（sol- gel CW 20M）SPE吸附剂和sol-gel CW 20M涂层的织物相吸附萃取（FPSE）介质，用于同时萃取和分析三种可乐性肠病（IBD）药物，其可的松的logP值（极性）为1.66，环丙沙星为2.30，柳氮磺吡啶为2.92。商业SPE相和内部合成的sol-gel CW 20M SPE相都装入SPE柱中，并且在详尽的萃取模式下进行萃取。 FPSE在平衡萃取模式下进行。使用Luna C18色谱柱（250 mm×4.6 mm； 5μm粒径）在20分钟内以梯度洗脱模式分离药物化合物，并根据国际生物分析方法验证指南对方法进行了验证。新型内部合成和加载的溶胶-凝胶CW 20M SPE吸附剂柱的特征在于其提取能力，突破体积，保留体积，保留体积，理论塔板数和保留因子。

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