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首页> 美国卫生研究院文献>Mycobiology >First Report of Potato Stem-End Rot Caused by Fusarium oxysporum in Korea
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First Report of Potato Stem-End Rot Caused by Fusarium oxysporum in Korea

机译：韩国首次报道由尖孢镰刀菌引起的马铃薯茎端腐烂

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In this study, we identified the causative agent of stem-end rot in potatoes that were grown in Gangwon alpine areas of Korea in 2013. The disease symptoms included appearance of slightly sunken circular lesion with corky rot on the potato surface at the stem-end portion. The fungal species isolated from the infected potatoes were grown on potato dextrose agar and produced white aerial mycelia with dark violet pigments. The conidiophores were branched and monophialidic. The microconidia had ellipsoidal to cylindrical shapes and ranged from 2.6~11.4 × 1.9~3.5 µm in size. The macroconidia ranged from 12.7~24.7 × 2.7~3.6 µm in size and had slightly curved or fusiform shape with 2 to 5 septate. Chlamydospores ranged from 6.1~8.1 × 5.7~8.3 µm in size and were present singly or in pairs. The causal agent of potato stem-end rot was identified as Fusarium oxysporum by morphological characterization and by sequencing the internal transcribed spacer (ITS1 and ITS4) regions of rRNA. Artificial inoculation of the pathogen resulted in development of disease symptoms and the re-isolated pathogen showed characteristics of F. oxysporum. To the best of our knowledge, this is the first study to report that potato stem-end rot is caused by F. oxysporum in Korea.
机译：在这项研究中，我们确定了2013年在韩国江原高山地区种植的马铃薯茎端腐烂的病原体。该病的症状包括茎端马铃薯表面出现轻微凹陷的圆形病斑并有腐烂腐烂一部分。从感染的马铃薯中分离出的真菌菌种在马铃薯葡萄糖琼脂上生长，并产生带有深紫色颜料的白色气生菌丝体。分生孢子是分枝的和单斜生的。微型分生孢子具有椭圆形至圆柱形的形状，尺寸为2.6〜11.4×1.9〜3.5 µm。大分生孢子体大小为12.7〜24.7×2.7〜3.6 µm，呈微弯曲或梭形，有2至5个隔室。衣原体的大小范围为6.1〜8.1×5.7〜8.3 µm，单独或成对存在。马铃薯茎端腐烂的病原体通过形态特征鉴定和对rRNA的内部转录间隔区（ITS1和ITS4）进行测序，确定为尖孢镰刀菌。病原体的人工接种导致疾病症状的发展，并且重新分离的病原体显示出尖孢镰刀菌的特征。据我们所知，这是第一个报道马铃薯茎端腐烂是由韩国产地孢霉引起的研究。

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