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首页> 美国卫生研究院文献>Mycobiology >Cloning and Expression Analysis of Phenylalanine Ammonia-Lyase Gene in the Mycelium and Fruit Body of the Edible Mushroom Flammulina velutipes
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Cloning and Expression Analysis of Phenylalanine Ammonia-Lyase Gene in the Mycelium and Fruit Body of the Edible Mushroom Flammulina velutipes

机译：食用菌金针菇菌丝体和子实体苯丙氨酸氨解酶基因的克隆与表达分析

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摘要

Phenylalanine ammonia-lyase (PAL) gene is known to be expressed in plants, and is involved in the differentiation, growth and synthesis of secondary metabolites. However, its expression in fungi remains to be explored. To understand its expression in mushroom fungi, the PAL gene of the edible mushroom Flammulina velutipes (Fvpal) was cloned and characterized. The cloned Fvpal consists of 2,175 bp, coding for a polypeptide containing 724 amino acids and having 11 introns. The translated amino acid sequence of Fvpal shares a high identity (66%) with that of ectomycorrhizal fungus Tricholoma matsutake. Distinctively, the Fvpal expression in the mycelium was higher in minimal medium supplemented with L-tyrosine than with other aromatic amino acids. During cultivation of the mushroom on sawdust medium, Fvpal expression in the fruit body correspondingly increased as the mushroom grew. In the fruiting body, Fvpal was expressed more in the stipe than in the pileus. These results suggest that F. velutipes PAL activity differs in the different organs of the mushroom. Overall, this is first report to show that the PAL gene expression is associated with mushroom growth in fungi.
机译：苯丙氨酸氨裂合酶（PAL）基因已知在植物中表达，并参与次级代谢产物的分化，生长和合成。然而，其在真菌中的表达仍有待探索。为了了解其在蘑菇真菌中的表达，克隆并鉴定了食用蘑菇金针菇（Fvpal）的PAL基因。克隆的Fvpal由2,175 bp组成，编码包含724个氨基酸和11个内含子的多肽。 Fvpal的翻译氨基酸序列与外生菌根真菌松茸（Tricholoma matsutake）具有高度同一性（66％）。明显地，在补充L-酪氨酸的基本培养基中，菌丝体中Fvpal的表达高于其他芳香族氨基酸。在木屑培养基上培养蘑菇的过程中，随着蘑菇的生长，子实体中Fvpal的表达相应增加。在子实体中，Fvpal在刀柄中比在绒头中表达更多。这些结果表明，金针菇的PAL活性在蘑菇的不同器官中是不同的。总体而言，这是第一个报告，表明PAL基因表达与真菌中的蘑菇生长有关。

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