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Synthesis of the human insulin gene. Part III. Chemical synthesis of 5-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA.

机译：人胰岛素基因的合成。第三部分通过修饰的磷酸三酯方法化学合成含5-磷酸单酯基的脱氧核糖寡核苷酸。它在合成构成人胰岛素C链DNA的十七个片段中的应用。

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摘要

A method for phosphorylating a protected deoxyribooligonucleotide containing phosphotriester linkages is described. The modified phosphotriester method of chemical synthesis is further refined in terms of (i) better final deblocking conditions and (ii) new chromatography solvent systems containing acetone-water-ethyl acetate to yield pure oligomers. The effectiveness of these improvements has been demonstrated in the rapid and efficient synthesis of seventeen fragments constituting the sequence of human insulin C-chain DNA.

机译：描述了一种使包含磷酸三酯键的保护的脱氧核糖寡核苷酸磷酸化的方法。根据（i）更好的最终解封条件和（ii）含有丙酮-水-乙酸乙酯的新色谱溶剂系统，可以得到更纯的低聚物，从而进一步改进了化学合成的改良磷酸三酯方法。这些改进的有效性已在构成人类胰岛素C链DNA序列的十七个片段的快速有效合成中得到了证明。

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期刊名称 Nucleic Acids Research
作者
H M Hsiung; W L Sung; R Brousseau; R Wu; S A Narang;
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作者单位

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年(卷),期 1980(8),23
年度 1980
页码 5753–5765
总页数 13
原文格式 PDF
正文语种
中图分类分子生物学;
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1. Synthesis of the human insulin gene. Part III1. Chemical synthesis of 5′-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA [J] . Hansen M. Hsiung, Ray Wu, Roland Brousseau, Nucleic acids research . 1980,第23期

机译：人胰岛素基因的合成。第三部分1。通过修饰的磷酸三酯方法化学合成含5'-磷酸单酯基的脱氧核糖寡核苷酸。它在合成构成人胰岛素C链DNA的十七个片段中的应用
2. Synthesis of the human insulin gene. Part II. Further improvements in the modified phosphotriester method and the synthesis of seventeen deoxyribooligonucleotide fragments constituting human insulin chains B and mini-C DNA [J] . Hansen M. Hsiung, Joseph Michniewicz, Ray Wu, Nucleic acids research . 1979,第8期

机译：人胰岛素基因的合成。第二部分改进的磷酸三酯方法的进一步改进以及构成人胰岛素链B和mini-C DNA的17个脱氧核糖寡核苷酸片段的合成
3. Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribo-oligonucleotides fragments constituting human insulin A DNA [J] . Hansen M. Hsiung, Joseph Michniewicz, Roland Brousseau, Nucleic acids research . 1979,第4期

机译：人胰岛素基因的合成。第一部分，改进的三酯法反相色谱的发展。它在快速有效合成组成人胰岛素A DNA的8个脱氧核糖寡核苷酸片段中的应用
4. Semi-synthesis of human Ghrelin:Combination of recombinant peptide synthesis with chemical synthesis of O-acylated fragment(1) [C] . Tomohiro Makino, Masaru Matsumoto, Yuji Suzuki International Peptide Symposium;European Peptide Symposium . 2005

机译：人类Ghrelin的半合成：重组肽合成与o-酰化片段的化学合成的组合（1）
5. Synthesis, characterization, and application of polyethylene glycol modified insulin for oral delivery using complexation hydrogels. [D] . Tuesca, Anthony D. 2008

机译：聚乙二醇修饰的胰岛素的合成，表征和应用，使用络合水凝胶进行口服给药。
6. Synthesis of human insulin gene. Part I. Development of reversed-phase chromatography in the modified triester method. Its application in the rapid and efficient synthesis of eight deoxyribooligonucleotides fragments constituting human insulin A DNA. [O] . H M Hsiung, R Brousseau, J Michniewicz, 1979

机译：人胰岛素基因的合成。第一部分改进的三酯法反相色谱的发展。它在快速有效地合成构成人胰岛素A DNA的八个脱氧核糖寡核苷酸片段中的应用。
7. Synthesis of the human insulin gene. Part III. Chemical synthesis of 5'-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA. [O] . Hsiung, H M, Sung, W L, Brousseau, R, 1980

机译：人胰岛素基因的合成。第三部分通过修饰的磷酸三酯方法化学合成含5'-磷酸单酯基的脱氧核糖寡核苷酸。它在合成构成人胰岛素C链DNA的十七个片段中的应用。
8. Chemical Modification of Matrix Resin Networks with Engineering Thermoplastics. III. Synthesis and Properties of Epoxy Networks Modified with Amine Terminated Poly(Aryl Ether Sulfone) Oligomers [R] . Hedrick, J. L., Jurek, M. J., Yilgor, I., 1985

机译：工程热塑性塑料对基质树脂网络的化学改性。 III。胺封端聚（芳醚砜）低聚物改性环氧网络的合成与性能

Synthesis of the human insulin gene. Part III. Chemical synthesis of 5-phosphomonoester group containing deoxyribooligonucleotides by the modified phosphotriester method. Its application in the synthesis of seventeen fragments constituting human insulin C-chain DNA.

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