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首页> 美国卫生研究院文献>Nucleic Acids Research >An elongated model of the Xenopus laevis transcription factor IIIA-5S ribosomal RNA complex derived from neutron scattering and hydrodynamic measurements.
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An elongated model of the Xenopus laevis transcription factor IIIA-5S ribosomal RNA complex derived from neutron scattering and hydrodynamic measurements.

机译：Xenopus laevis转录因子IIIA-5S核糖体RNA复合物的细长模型其源自中子散射和流体动力学测量。

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The precise molecular composition of the Xenopus laevis TFIIIA-5S ribosomal RNA complex (7S particle) has been established from small angle neutron and dynamic light scattering. The molecular weight of the particle was found to be 95,700 +/- 10,000 and 86,700 +/- 9000 daltons from these two methods respectively. The observed match point of 54.4% D2O obtained from contrast variation experiments indicates a 1:1 molar ratio. It is concluded that only a single molecule of TFIIIA, a zinc-finger protein, and of 5S RNA are present in this complex. At high neutron scattering contrast radius of gyration of 42.3 +/- 2 A was found for the 7S particle. In addition a diffusion coefficient of 4.4 x 10(-11) [m2 s-1] and a sedimentation coefficient of 6.2S were determined. The hydrodynamic radius obtained for the 7S particle is 48 +/- 5 A. A simple elongated cylindrical model with dimensions of 140 A length and 59 A diameter is compatible with the neutron results. A globular model can be excluded by the shallow nature of the neutron scattering curves. It is proposed that the observed difference of 15 A in length between the 7S particle and isolated 5S RNA most likely indicates that part(s) of the protein protrudes from the end(s) of the RNA molecule. There is no biochemical evidence for any gross alteration in 5S RNA conformation upon binding to TFIIIA.
机译：非洲爪蟾TFIIIA-5S核糖体RNA复合物（7S颗粒）的精确分子组成是通过小角度中子和动态光散射建立的。通过这两种方法，发现该颗粒的分子量分别为95,700 +/- 10,000和86,700 +/- 9000道尔顿。从对比度变化实验获得的观察到的匹配点为54.4％D2O，表明摩尔比为1：1。结论是，该复合物中仅存在TFIIIA，锌指蛋白和5S RNA的单个分子。在高中子散射下，发现7S粒子的旋转对比度反差半径为42.3 +/- 2A。另外，确定的扩散系数为4.4 x 10（-11）[m2 s-1]，沉降系数为6.2S。对于7S粒子获得的流体力学半径为48 +/- 5A。一个简单的细长圆柱模型（长度140 A，直径59 A）与中子结果兼容。中子散射曲线的浅层性质可以排除球状模型。提出观察到的7S颗粒和分离的5S RNA之间15 A的长度差异最有可能表明蛋白质的一部分从RNA分子的末端突出。没有任何生化证据表明与TFIIIA结合后5S RNA构象发生任何重大改变。

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