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Preparation of a novel psoralen containing deoxyadenosine building block for the facile solid phase synthesis of psoralen-modified oligonucleotides for a sequence specific crosslink to a given target sequence.

机译：制备新颖的含有脱氧腺苷构建基的补骨脂素用于容易地固相合成补骨脂素修饰的寡核苷酸以实现与给定靶序列的序列特异性交联。

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摘要

4,5',8-Trimethylpsoralen was attached to the C8-position of deoxyadenosine via a sulfur atom and a five carbon atom linker. The modified deoxyadenosine was then converted to a protected phosphoramidite and used as unusual as a building block for solid phase oligodeoxyribonucleotide synthesis. The efficiency of the photoreaction of a psoralen-modified oligonucleotide to a complementary matrix strand reached more than 90% within a 1 hour irradiation time at a wavelength of 345 nm.

机译：4,5'，8-三甲基补骨脂素通过一个硫原子和一个五个碳原子的连接基连接于脱氧腺苷的C8位。然后将修饰的脱氧腺苷转化为受保护的亚磷酰胺，并作为固相寡聚脱氧核糖核苷酸合成的常规成分使用。补骨脂素修饰的寡核苷酸对互补基质链的光反应效率在1小时的照射时间内在345 nm波长下达到90％以上。

著录项

期刊名称 Nucleic Acids Research
作者
U Pieles; B S Sproat; P Neuner; F Cramer;
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作者单位

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年(卷),期 1989(17),22
年度 1989
页码 8967–8978
总页数 12
原文格式 PDF
正文语种
中图分类分子生物学;
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1. Preparation of a novel psoralen containing deoxyadenosine building block for the facile solid phase synthesis of psoralen-modified oligonucleotides for a sequence specific crosslink to a given target sequence [J] . B.S. Sproat, F. Cramer, P. Neuner, Nucleic acids research . 1989,第22期

机译：制备新颖的含脱氧腺苷补骨脂素的补骨脂素，用于补骨脂蛋白修饰的寡核苷酸的固相合成，以实现与给定靶序列的序列特异性交联
2. Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: characterization of psoralen monoadduct and crosslink formation [J] . Edward J. Gunther, Francis P. Gasparro, Gerard A. Olack, Nucleic acids research . 1994,第14期

机译：用三螺旋形成的寡核苷酸定点靶向补骨脂素光加合物：补骨脂素单加合物的特征和交联形成
3. Enzymatic synthesis of oligonucleotides of defined sequence. Addition of short blocks of nucleotide residues to oligonucleotide primers [J] . Kimberley Waterman, Michael Smith, Shirley Gillam Nucleic acids research . 1975,第5期

机译：酶促合成确定序列的寡核苷酸。向寡核苷酸引物添加核苷酸残基的短嵌段
4. Sequence-specific photoinduced c-fos gene damage mediated by triple stranded-forming oligonucleotide conjugated to psoralen [C] . Enhua Cao, Institute of Biophysics, Beijing, 1999 International Conference on Biomedical Optics . 1999

机译：补骨脂素结合的三链寡核苷酸介导的序列特异性光诱导的c-fos基因损伤
5. Triplex-forming oligonucleotide directed psoralen interstrand crosslinks can induce targeted homologous recombination in mammalian cells. [D] . Liu, Yaobin. 2009

机译：形成三链体的寡核苷酸定向的补骨脂素链间交联可以在哺乳动物细胞中诱导靶向的同源重组。
6. Site-specific targeting of psoralen photoadducts with a triple helix-forming oligonucleotide: characterization of psoralen monoadduct and crosslink formation. [O] . F P Gasparro, P A Havre, G A Olack, 1994

机译：用三重螺旋形成寡核苷酸对补骨脂素光加合物的位点特异性靶向：补骨脂素单加合物的特征和交联形成。
7. Preparation of a novel psoralen containing deoxyadenosine building block for the facile solid phase synthesis of psoralen-modified oligonucleotides for a sequence specific crosslink to a given target sequence. [O] . Pieles, U, Sproat, B S, Neuner, P, 1989

机译：制备新颖的含有脱氧腺苷构建基的补骨脂素，用于容易地固相合成补骨脂素修饰的寡核苷酸，以实现与给定靶序列的序列特异性交联。

Preparation of a novel psoralen containing deoxyadenosine building block for the facile solid phase synthesis of psoralen-modified oligonucleotides for a sequence specific crosslink to a given target sequence.

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