首页> 美国卫生研究院文献>Nucleic Acids Research >U21 a novel small nucleolar RNA with a 13 nt. complementarity to 28S rRNA is encoded in an intron of ribosomal protein L5 gene in chicken and mammals.

【2h】

U21 a novel small nucleolar RNA with a 13 nt. complementarity to 28S rRNA is encoded in an intron of ribosomal protein L5 gene in chicken and mammals.

机译：U21具有13 nt的新型小核仁RNA。与28S rRNA的互补性在鸡和哺乳动物的核糖体蛋白L5基因的内含子中编码。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

Following a search of sequence data bases for intronic sequences exhibiting structural features typical of snoRNAs, we have positively identified by Northern assays and sequence analysis another intron-encoded snoRNA, termed U21. U21 RNA is a 93 nt. long, metabolically stable RNA, present at about 10(4) molecules per HeLa cell. It is encoded in intron 5 of the ribosomal protein L5 gene, both in chicken and in the two mammals studied so far, human and mouse. U21 RNA is devoid of a 5'-trimethyl-cap and is likely to result from processing of intronic RNA. The nucleolar localization of U21 has been established by fluorescence microscopy after in situ hybridization with digoxigenin-labeled oligonucleotide probes. Like most other snoRNAs U21 contains the box C and box D motifs and is precipitated by anti-fibrillarin antibodies. By the presence of a typical 5'-3' terminal stem, U21 appears more particularly related to U14, U15, U16 and U20 intron-encoded snoRNAs. Remarkably, U21 contains a long stretch (13 nt.) of complementarity to a highly conserved sequence in 28S rRNA. Sequence comparisons between chicken and mammals, together with Northern hybridizations with antisense oligonucleotides on cellular RNAs from more distant vertebrates, point to the preferential preservation of this segment of U21 sequence during evolution. Accordingly, this complementarity, which overlaps the complementarity of 28S rRNA to another snoRNA, U18, could reflect an important role of U21 snoRNA in the biogenesis of large ribosomal subunit.

机译：在序列数据库中搜索到表现出snoRNA典型结构特征的内含子序列后，我们通过Northern分析和序列分析已确定地鉴定了另一种内含子编码的snoRNA，称为U21。 U21 RNA是93 nt。长的，代谢稳定的RNA，每个HeLa细胞约有10（4）个分子。它被编码在核糖体蛋白L5基因的5号内含子中，在鸡和迄今为止研究的两种哺乳动物中，无论是人类还是小鼠。 U21 RNA没有5'-三甲基帽，可能是内含子RNA加工产生的。与洋地黄毒苷标记的寡核苷酸探针原位杂交后，已通过荧光显微镜确定了U21的核仁定位。像大多数其他snoRNA一样，U21包含C框和D框基元，并由抗原纤维蛋白抗体沉淀。由于存在典型的5'-3'末端茎，U21似乎更特别地与U14，U15，U16和U20内含子编码的snoRNA相关。值得注意的是，U21与28S rRNA中高度保守的序列具有很长的互补性（13 nt。）。鸡与哺乳动物之间的序列比较，以及与来自更遥远脊椎动物的细胞RNA上的反义寡核苷酸进行Northern杂交，都表明在进化过程中优先保留了U21序列的这一片段。因此，这种互补性与28S rRNA与另一种snoRNA U18的互补性重叠，可能反映了U21 snoRNA在大核糖体亚基生物发生中的重要作用。

著录项

期刊名称 Nucleic Acids Research
作者
L H Qu; M Nicoloso; B Michot; M C Azum; M Caizergues-Ferrer; M H Renalier; J P Bachellerie;
展开▼
作者单位

展开▼
年(卷),期 1994(22),20
年度 1994
页码 4073–4081
总页数 9
原文格式 PDF
正文语种
中图分类分子生物学;
关键词

相似文献

外文文献
中文文献
专利

1. U21, a novel small nucleolar RNA with a 13 nt. complementarity to 28S rRNA, is encoded in an intron of ribosomal protein L5 gene in chicken and mammals [J] . Bernard Michot, Jean-Pierre Bachellerie, Liang-Hu Qu, Nucleic acids research . 1994,第20期

机译：U21，具有13 nt的新型小核仁RNA。与28S rRNA的互补性，是在鸡和哺乳动物的核糖体蛋白L5基因的内含子中编码的
2. U24, A NOVEL INTRON-ENCODED SMALL NUCLEOLAR RNA WITH TWO 12 NT LONG, PHYLOGENETICALLY CONSERVED COMPLEMENTARITIES TO 28S RRNA [J] . Qu LH, Henry Y, Nicoloso M, Nucleic Acids Research . 1995,第14期

机译：U24，一种新的内含子编码的小核仁RNA，具有两个12 NT长，与28S RRNA的同源性
3. U17XS8, a small nucleolar RNA with a 12 nt complementarity to 18S rRNA and coded by a sequence repeated in the six introns of Xenopus laevis ribosomal protein S8 gene [J] . Fabrizio Loreni, Francesco Amaldi, Francesco Cecconi, Nucleic acids research . 1994,第5期

机译：U17XS8，与18S rRNA具有12 nt互补性的小核仁RNA，由非洲爪蟾核糖体蛋白S8基因的六个内含子重复的序列编码
4. Interaction Form between Splided Introns and Corresponding Protein Coding Sequences of Ribosomal Protein Genes in C.elegans [C] . ZHAO Xiaoqing, LI Hong, Yang Linyuan 4th Chinese conference on bioinformatics and systems biology. . 2010

机译：秀丽隐杆线虫内含子内含子与核糖体蛋白基因相应蛋白编码序列的相互作用形式
5. The molecular phylogeny of Stomatopod crustaceans inferred using combined COI, 18s and 28s ribosomal RNA genes and a study of the evolution of stomatopod compound eye [D] . Zhang, Yunfei 2009

机译：利用COI，18s和28s核糖体RNA基因组合推论甲壳纲动物的分子系统发育及对角膜足动物进化的研究
6. U24 a novel intron-encoded small nucleolar RNA with two 12 nt long phylogenetically conserved complementarities to 28S rRNA. [O] . L H Qu, Y Henry, M Nicoloso, 1995

机译：U24一种新颖的内含子编码小核仁RNA具有两个12 nt长在系统发育上与28S rRNA互补。
7. U21, a novel small nucleolar RNA with a 13 nt. complementarity to 28S rRNA, is encoded in an intron of ribosomal protein L5 gene in chicken and mammals. [O] . Qu, L H, Nicoloso, M, Michot, B, 1994

机译：U21，具有13 nt的新型小核仁RNA。与28S rRNA的互补性在鸡和哺乳动物的核糖体蛋白L5基因的内含子中编码。

U21 a novel small nucleolar RNA with a 13 nt. complementarity to 28S rRNA is encoded in an intron of ribosomal protein L5 gene in chicken and mammals.

摘要

著录项

相似文献

相关主题

期刊订阅