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Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays

机译：杂交动力学用于区分对寡核苷酸微阵列的特异性与非特异性结合的方法

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摘要

Hybridization kinetics were found to be significantly different for specific and non-specific binding of labeled cRNA to surface-bound oligonucleotides on microarrays. We show direct evidence that in a complex sample specific binding takes longer to reach hybridization equilibrium than the non- specific binding. We find that this property can be used to estimate and to correct for the hybridization contributed by non-specific binding. Useful applications are illustrated including the selection of superior oligonucleotides, and the reduction of false positives in exon identification.

机译：发现杂交动力学对于标记的cRNA与微阵列上的表面结合的寡核苷酸的特异性和非特异性结合显着不同。我们显示出直接的证据，在复杂的样品中，特异性结合比非特异性结合需要更长的时间才能达到杂交平衡。我们发现该性质可用于估计和校正由非特异性结合贡献的杂交。说明了有用的应用，包括选择优良的寡核苷酸，以及减少外显子鉴定中的假阳性。

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期刊名称 Nucleic Acids Research
作者
Hongyue Dai; Michael Meyer; Sergey Stepaniants; Michael Ziman; Roland Stoughton;
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作者单位

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年(卷),期 2002(30),16
年度 2002
页码 e86
总页数 8
原文格式 PDF
正文语种
中图分类分子生物学;
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专利

1. Use of hybridization kinetics for differentiating specific from non‐specific binding to oligonucleotide microarrays [J] . Hongyue Dai, Michael Meyer, Michael Ziman, Nucleic acids research . 2002,第16期

机译：利用杂交动力学区分寡核苷酸微阵列的特异性结合与非特异性结合
2. Model-based analysis of non-specific binding for background correction of high-density oligonucleotide microarrays [J] . Furusawa Chikara, Ono Naoaki, Suzuki Shingo, Bioinformatics . 2009,第1期

机译：基于模型的非特异性结合的背景分析，用于高密度寡核苷酸微阵列的背景校正
3. Model-based analysis of non-specific binding for background correction of high-density oligonucleotide microarrays [J] . Chikara Furusawa12*† Naoaki Ono1† Shingo Suzuki1 Tomoharu Agata1 Hiroshi Shimizu1 and Tetsuya Yomo123 Bioinformatics . 2009,第1期

机译：基于模型的非特异性结合的背景分析，用于高密度寡核苷酸微阵列的背景校正
4. Minimizing DNA microarrays to a single molecule per spot: using zero-mode waveguide technology to obtain kinetic data for a large number of short oligonucleotide hybridization reactions [C] . Jens Sobek, Hubert Rehrauer, Gerrit Kuhn, Conference on frontiers in biological detection: from nanosensors to systems . 2016

机译：将每个点的DNA微阵列最小化为单个分子：使用零模式波导技术获得大量短寡核苷酸杂交反应的动力学数据
5. Protein binding microarrays for the comprehensive characterization of transcription factor binding specificities. [D] . Berger, Michael Forman. 2008

机译：蛋白结合微阵列，用于全面表征转录因子结合特异性。
6. Effects of Target Length on the Hybridization Efficiency and Specificity of rRNA-Based Oligonucleotide Microarrays [O] . Wen-Tso Liu, Huiling Guo, Jer-Horng Wu 2007

机译：目标长度对基于rRNA的寡核苷酸芯片的杂交效率和特异性的影响。
7. Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays [O] . Dai, Hongyue, Meyer, Michael, Stepaniants, Sergey, 2002

机译：杂交动力学用于区分对寡核苷酸微阵列的特异性与非特异性结合的方法

Use of hybridization kinetics for differentiating specific from non-specific binding to oligonucleotide microarrays

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