首页> 美国卫生研究院文献>Journal of Virology >Early gene expression in bacteriophage T7. I. In vivo synthesis inactivation and translational utilization of early mRNAs.
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Early gene expression in bacteriophage T7. I. In vivo synthesis inactivation and translational utilization of early mRNAs.

机译:T7噬菌体中的早期基因表达。 I.早期mRNA的体内合成失活和翻译利用。

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摘要

In vivo decay rates for the individual T7 early mRNA species were determined. The physical half-lives, measured at 37 C, range from 1.1 min for gene 0.7 RNA to 4.5 min for gene 0.3 RNA. Physical half-lives, as observed after rifampin inhibition of RNA synthesis and polyacylamide electrophoresis of RNAs, are approximately 30% longer than functional half-lives, as observed by 14C-labeled amino acid uptake into individual T7 early proteins. The different RNA species are synthesized at grossly different rates, 0.3 RNA at four times the rate of 1.0 RNA, 0.7 RNA at twice the rate, and 1.1 and 1.3 RNAs at about the same or a slightly lower rate than 1.0 RNA. Rho-factor-mediated termination of transcription behind genes 0.3, 0.7, and perhaps behind 1.0 is inferred from these data. The in vivo translational utilization of the individual T7 early-message species was found to vary by not more than a factor of 2.
机译:确定了各个T7早期mRNA种类的体内衰变率。在37 C下测得的物理半衰期,从基因0.7 RNA的1.1分钟到基因0.3 RNA的4.5分钟不等。利福平抑制RNA合成和RNA的聚丙烯酰胺电泳后观察到的物理半衰期比功能性半衰期长约30%,如14C标记的氨基酸吸收到单个T7早期蛋白质中所观察到的。不同的RNA种类以完全不同的速率合成,0.3 RNA的合成速率是1.0 RNA的四倍,0.7 RNA的合成速率是1.0 RNA的两倍,而1.1和1.3 RNA的合成速率与1.0 RNA大致相同或略低。从这些数据可以推断出Rho因子介导的基因0.3、0.7甚至是1.0之后的转录终止。发现单个T7早期消息种类的体内翻译利用变化不超过2倍。

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