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首页> 美国卫生研究院文献>Journal of Virology >Evolutionary relationships of the primate papovaviruses: base sequence homology among the genomes of simian virus 40 stump-tailed macaque virus and SA12 virus.
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Evolutionary relationships of the primate papovaviruses: base sequence homology among the genomes of simian virus 40 stump-tailed macaque virus and SA12 virus.

机译：灵长类乳头状病毒的进化关系：猿猴病毒40残尾猕猴和SA12病毒基因组之间的碱基序列同源性。

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Physical maps of the genomes of the two newly discovered primate papovaviruses, SA12 and stump-tailed macaque virus (STMV), were generated by restriction endonuclease analysis. The base sequence homologies among the genomes of SA12, stump-tailed macaque virus, and simian virus 40 (SV40) were studied by heteroduplex analysis. Heteroduplexes between SA12 and SV40 DNAs and stump-tailed macaque virus and SV40 DNAs were constructed and mounted for electron microscopy in various amounts of formamide to achieve a range of effective temperatures. At each effective temperature, the regions of duplex DNA in the heteroduplexes were measured and localized on the SV40 physical and functional maps. By analyzing the data from this study and rom our previous study (N. Newell, C. J. Lai, G. Khoury, and T. J. Kelly Jr., J. Virol. 25:193-201, 1978) on the base sequence homology between the genomes of BK virus and SV40, some general conclusions have been drawn concerning the evolutionary relationships among the genomes of the primate papovaviruses. The extent of homology among the viral genomes does not reflect the phylogenetic relationships of their hosts. At comparable effective temperatures Tm - 33 degrees C), the heteroduplexes between the DNAs of BK virus and SV40 contained the largest amount of duplex (about 90%). The heteroduplexes made between SA12 and SV40 DNAs were slightly less homologous, containing about 80% duplex. The heteroduplexes made between SV40 and stump-tailed macaque virus DNAs were only 20% duplex under the same conditions. When the various heteroduplexes were mounted for microscopy at effective temperatures greater than Tm - 33 degrees C, the fraction of the duplex DNA decreased in each case, indicating the existence of considerable base mismatching in the homologous regions. When specific coding or noncoding regions of the viral genomes were compared, the data indicated that the extent of sequence divergence differed markedly from one region to another. In all the heteroduplexes studied, there were two regions, located near the junctions between early and late regions on the SV40 map, which were essentially nonhomologous. All of the heteroduplexes studied showed significantly greater homology in the late region than in early region. Within the late region, the sequences coding for the major capsid polypeptide, VP1, were the most highly conserved.
机译：通过限制性核酸内切酶分析生成了两种新近发现的灵长类乳头状病毒SA12和残尾猕猴（STMV）的基因组的物理图谱。通过异源双链分析，研究了SA12，树桩尾猕猴病毒和猿猴病毒40（SV40）基因组之间的碱基序列同源性。构建SA12和SV40 DNA与残尾猕猴病毒和SV40 DNA之间的异源双链体，并以各种数量的甲酰胺进行电子显微镜检查，以实现一系列有效温度。在每个有效温度下，测量异源双链体中双链体DNA的区域，并将其定位在SV40物理和功能图上。通过分析本研究和我们以前的研究（N. Newell，CJ Lai，G。Khoury和TJ Kelly Jr.，J. Virol。25：193-201，1978）中有关基因组之间碱基序列同源性的数据关于BK病毒和SV40，关于灵长类乳头状病毒的基因组之间的进化关系已得出一些一般性结论。病毒基因组之间的同源性程度不能反映其宿主的系统发育关系。在相当的有效温度Tm-33摄氏度）下，BK病毒和SV40的DNA之间的异源双链体包含最大数量的双链体（约90％）。 SA12和SV40 DNA之间产生的异源双链体同源性略低，包含约80％的双链体。在相同条件下，SV40与残尾猕猴病毒DNA之间形成的异源双链体只有20％的双链体。当在高于Tm-33摄氏度的有效温度下安装各种异源双链体进行显微镜检查时，双链体DNA的比例在每种情况下均下降，这表明在同源区域中存在相当大的碱基错配。当比较病毒基因组的特定编码区或非编码区时，数据表明序列差异的程度在一个区域与另一个区域之间显着不同。在所有研究的异源双链体中，有两个区域位于SV40图上早期区域和晚期区域之间的交点附近，这两个区域基本上是非同源的。研究的所有异源双链体在晚期区域均显示出比早期区域更大的同源性。在晚期区域，编码主要衣壳多肽VP1的序列是最保守的。

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