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首页> 外文期刊>Journal of Virology >Evolutionary relationships of the primate papovaviruses: base sequence homology among the genomes of simian virus 40, stump-tailed macaque virus, and SA12 virus.
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Evolutionary relationships of the primate papovaviruses: base sequence homology among the genomes of simian virus 40, stump-tailed macaque virus, and SA12 virus.

机译：灵长类动物乳头病毒的进化关系：猿猴病毒40，树桩尾猕猴及SA12病毒基因组中基因序列同源性。

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Physical maps of the genomes of the two newly discovered primate papovaviruses, SA12 and stump-tailed macaque virus (STMV), were generated by restriction endonuclease analysis. The base sequence homologies among the genomes of SA12, stump-tailed macaque virus, and simian virus 40 (SV40) were studied by heteroduplex analysis. Heteroduplexes between SA12 and SV40 DNAs and stump-tailed macaque virus and SV40 DNAs were constructed and mounted for electron microscopy in various amounts of formamide to achieve a range of effective temperatures. At each effective temperature, the regions of duplex DNA in the heteroduplexes were measured and localized on the SV40 physical and functional maps. By analyzing the data from this study and rom our previous study (N. Newell, C. J. Lai, G. Khoury, and T. J. Kelly Jr., J. Virol. 25:193-201, 1978) on the base sequence homology between the genomes of BK virus and SV40, some general conclusions have been drawn concerning the evolutionary relationships among the genomes of the primate papovaviruses. The extent of homology among the viral genomes does not reflect the phylogenetic relationships of their hosts. At comparable effective temperatures Tm - 33 degrees C), the heteroduplexes between the DNAs of BK virus and SV40 contained the largest amount of duplex (about 90%). The heteroduplexes made between SA12 and SV40 DNAs were slightly less homologous, containing about 80% duplex. The heteroduplexes made between SV40 and stump-tailed macaque virus DNAs were only 20% duplex under the same conditions. When the various heteroduplexes were mounted for microscopy at effective temperatures greater than Tm - 33 degrees C, the fraction of the duplex DNA decreased in each case, indicating the existence of considerable base mismatching in the homologous regions. When specific coding or noncoding regions of the viral genomes were compared, the data indicated that the extent of sequence divergence differed markedly from one region to another. In all the heteroduplexes studied, there were two regions, located near the junctions between early and late regions on the SV40 map, which were essentially nonhomologous. All of the heteroduplexes studied showed significantly greater homology in the late region than in early region. Within the late region, the sequences coding for the major capsid polypeptide, VP1, were the most highly conserved.
机译：通过限制性内切核酸酶分析产生两种新发现的灵长类动物乳头病毒，SA12和树桩尾猕猴（STMV）的基因组的物理图。通过异络分析研究了SA12，树桩尾部猕猴病毒和Simian病毒40（SV40）的基因组中的碱基序列同源物。在SA12和SV40DNA和树脂尾部猕猴和SV40DNA之间的异熔性构造和安装在各种量的甲酰胺中的电子显微镜，以实现一系列有效的温度。在每个有效温度下，在SV40物理和功能图上测量并局限性化合物中双相DNA的区域。通过分析本研究和ROM的数据我们以前的研究（N. Newell，CJ Lai，G.Khoury，以及TJ Kelly Jr.，J.Virol。25：193-201,1978）在基因组之间的基本序列同源性上BK病毒和SV40，已经提出了一些一般的结论，关于灵长类动物乳头病毒基因组的进化关系。病毒基因组中的同源性的程度不反映其宿主的系统发育关系。在相当的有效温度Tm - 33摄氏度下，BK病毒和SV40的DNA之间的异熔性含有最大的双链体积（约90％）。在SA12和SV40 DNA之间制备的异熔相均略低于同源较小，含有约80％双链体。在SV40和树桩尾部猕猴病毒DNA之间制备的异渗在在相同条件下仅为20％双链体。当在大于Tm-33摄氏度的有效温度下安装各种异络合物以进行显微镜检查时，在每种情况下，双相DNA的分数减小，表明在同源区域中存在相当大的基础。当比较病毒基因组的特定编码或非编码区域时，数据表明序列发散程度从一个区域与另一个区域显着不同。在研究的所有异络合物中，有两个地区，位于SV40地图上的早期和后期区域之间的接线处，基本上是非博学的。所研究的所有异络合物在晚期的后期表现出显着更大的同源性。在后期区域内，编码主要衣壳多肽VP1的序列是最受保存的。

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