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首页> 美国卫生研究院文献>Journal of Virology >Detection of feline immunodeficiency virus (FIV) nucleic acids in FIV-seronegative cats.
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Detection of feline immunodeficiency virus (FIV) nucleic acids in FIV-seronegative cats.

机译：检测FIV血清阴性猫中的猫免疫缺陷病毒（FIV）核酸。

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A study was undertaken to determine the rate of viral transmission among naive specific-pathogen-free (SPF) cats living in close contact with feline immunodeficiency virus (FIV)-infected cats. Twenty SPF cats were housed in the same rooms with experimentally FIV-infected seropositive and virus culture-positive cats for 2 to 4 years and were monitored for the presence of FIV nucleic acids and antibodies. Only 1 of the 20 cats became seropositive and virus culture positive and developed signs of disease. Genomic DNA from bone marrow and peripheral blood mononuclear cells (PBMC) of 10 of 19 healthy-appearing seronegative cats became positive for FIV DNA by the polymerase chain reaction. Twenty-eight SPF cats housed as groups in separate quarters and never exposed to FIV-infected cats were uniformly negative for FIV DNA. FIV RNA transcripts were detected in concanavalin A-stimulated PBMC cultures from 4 of 10 FIV DNA-positive, seronegative cats by in situ hybridization. PBMC from three of four naive SPF cats acquired FIV nucleic acids after the cats were transfused with blood and bone marrow from FIV genome-positive, seronegative donors. Three of five FIV-seronegative cats housed for years with naturally FIV-infected cats in a private household were also found to harbor FIV DNA, indicating that the same phenomenon occurred in the field. These findings demonstrate that cats living in close contact with FIV-infected seropositive cats can acquire FIV nucleic acids without developing detectable levels of serum antibodies or disease.
机译：进行了一项研究，以确定与猫免疫缺陷病毒（FIV）感染的猫密切接触的幼稚的无特定病原体（SPF）猫之间的病毒传播速率。将20只SPF猫与实验性感染FIV的血清阳性和病毒培养阳性的猫一起饲养在同一房间中2至4年，并监测FIV核酸和抗体的存在。 20只猫中只有1只呈血清阳性，病毒培养呈阳性，并出现疾病迹象。通过聚合酶链反应，来自19只健康出现的血清阴性猫中的10只的骨髓和外周血单核细胞（PBMC）的基因组DNA对FIV DNA呈阳性。 28只SPF猫在不同的地方成组饲养，并且从未接触过FIV感染的猫的FIV DNA均呈阴性。通过原位杂交从10只FIV DNA阳性，血清阴性的猫中的4只在伴刀豆球蛋白A刺激的PBMC培养物中检测到FIV RNA转录本。在四只幼稚的SPF猫中，三只的PBMC用FIV基因组阳性，血清阴性供体的血液和骨髓进行输血后获得了FIV核酸。在私人家庭中与自然感染FIV的猫一起饲养多年的五只FIV血清阴性猫中，有三只也带有FIV DNA，这表明该现象在田间也发生了。这些发现表明，与FIV感染的血清反应阳性猫密切接触的猫可以获取FIV核酸，而不会产生可检测水平的血清抗体或疾病。

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