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Pollen grains as a target for introduction of foreign genes into plants: an assessment

机译：花粉粒作为将外源基因导入植物的靶标：一项评估

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Introduction of foreign genes and development of transgenic plants have become an integral part of crop improvement programmes in the last decade. However, most of the present day plant transformation protocols require long periods for development of transgenic plants and need skilled personnel. Development of alternate, simple and rapid transformation protocols for development of transgenic plants can overcome the constraints of in vitro culture, regeneration and associated problems. Pollen grains, due to their abundance and ease with which they can be handled are ideal targets for introduction of foreign genes into the germ line. However, progress in introduction of transgenes into pollen grains and their subsequent use in fertilization leading to development of transgenic plants are limited. With the recent progress made in understanding of pollen development along with reports of successful pollen-mediated transformation in important crop plants, it should be possible to extend this simple method of transformation to other crop plants. The review deals with development of pollen grains as a target for introduction of genes with special emphasis on recent developments.
机译：在过去的十年中，外来基因的导入和转基因植物的发展已成为作物改良计划不可或缺的一部分。然而，当今大多数植物转化方案需要长时间来发展转基因植物并且需要熟练的人员。开发用于转基因植物的替代，简单和快速的转化方案可以克服体外培养，再生和相关问题的限制。花粉粒由于其含量丰富且易于处理而成为将外源基因引入种系的理想靶标。然而，将转基因引入花粉粒中及其在随后的受精中导致转基因植物发育的进展有限。随着对花粉发育理解的最新进展以及重要农作物中花粉介导的成功转化的报道，应该有可能将此简单的转化方法扩展到其他农作物。该综述将花粉粒的发育作为引入基因的目标，特别强调了最近的发展。

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