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首页> 美国卫生研究院文献>Physiology and Molecular Biology of Plants >Utilization of in silico EST–SSR markers for diversity studies in castor (Ricinus communis L.)
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Utilization of in silico EST–SSR markers for diversity studies in castor (Ricinus communis L.)

机译：硅EST-SSR标记在蓖麻（Ricinus communis L.）多样性研究中的应用

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Castor (Ricinus communis L.) a chief non-edible oilseed crop has numerous industrial applications. Systematic genetic diversity analysis utilizing DNA based markers has been quick and reliable method that ensures selection of diverse parents for exploitation of higher levels of heterosis in breeding programs. From NCBI database, 63,852 EST sequences of castor were mined. One thousand one hundred and five (1105) EST–SSRs and 1652 repeat motifs sequences were identified from 20,495 non-redundant unigene sequences. Repeat motifs consisted of 29.7 % mono nucleotide repeats, 24.8 % di nucleotide repeats, 27.27 % tri nucleotide repeats and 3.94 % tetra nucleotide repeats. Twenty eight primer pairs were chosen from SSR-containing ESTs to determine genetic diversity among 27 castor accessions. Twelve EST–SSRs showed polymorphism. Number of alleles detected were 2–3 with an average of 2.33 per locus. 150–400 bp was the size of an allele. Dendrogram analysis grouped the 27 accessions into two separate clusters. Genetic similarity coefficient of dendrogram ranged from 0.24 to 0.83. The polymorphic information content value of 0.28–0.49 revealed medium level of diversity in castor. Results of present study indicated that EST–SSRs to be efficient markers for genetic diversity studies. Knowledge on level of diversity existing in castor genotypes would be useful for breeders to plan efficient hybrid breeding programme.Electronic supplementary materialThe online version of this article (doi:10.1007/s12298-016-0367-x) contains supplementary material, which is available to authorized users.
机译：蓖麻（Ricinus communis L.）是一种主要的非食用油料作物，具有许多工业应用。利用基于DNA的标记进行系统的遗传多样性分析是一种快速可靠的方法，可确保选择不同的亲本，以便在育种计划中利用更高水平的杂种优势。从NCBI数据库中，提取了63,852个蓖麻的EST序列。从20,495个非冗余单基因序列中鉴定了1,105个（1105）EST-SSR和1652个重复基序序列。重复基序由29.7％的单核苷酸重复，24.8％的二核苷酸重复，27.27％的三核苷酸重复和3.94％的四核苷酸重复组成。从含SSR的EST中选择了28对引物，以确定27个蓖麻种质之间的遗传多样性。十二个EST–SSRs显示出多态性。检测到的等位基因数量为2-3，每个基因座平均2.33。等位基因大小为150–400 bp。树状图分析将这27个种质分为两个单独的簇。树状图的遗传相似系数在0.24至0.83之间。多态信息含量值为0.28–0.49，表明蓖麻的中等多样性。目前的研究结果表明，EST-SSRs是遗传多样性研究的有效标记。有关蓖麻基因型中存在的多样性水平的知识将有助于育种者计划有效的杂交育种计划。电子补充材料本文的在线版本（doi：10.1007 / s12298-016-0367-x）包含补充材料，可用于授权用户。

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