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首页> 美国卫生研究院文献>Physiology and Molecular Biology of Plants >Interplay of genes in plant–pathogen interactions: In planta expression and docking studies of a beta 13 glucanase gene from Piper colubrinum and a glucanase inhibitor gene from Phytophthora capsici
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Interplay of genes in plant–pathogen interactions: In planta expression and docking studies of a beta 13 glucanase gene from Piper colubrinum and a glucanase inhibitor gene from Phytophthora capsici

机译：基因在植物与病原体相互作用中的相互作用：在植物的表达和对接研究中对来自Piper colubrinum的β13葡聚糖酶基因和来自疫霉菌的葡聚糖酶抑制剂基因的对接研究

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摘要

Oomycete pathogen, Phytophthora capsici is devastating for black pepper (Piper nigrum L.) and causes foot rot disease at all stages of plant growth. Phytophthora secretes a glucanase inhibitor protein (GIP), which is capable of inhibiting defence proteins like endoglucanases. In this particular study Quantitative PCR analysis, molecular docking studies and analysis of sequences of Glucanase inhibitor protein and beta-1,3 glucanse genes were done mainly depending on the data derived from Phytophthora capsici whole genome sequencing and Piper colubrinum RNA-sequencing (RNA-Seq). Amino acid sequence length of GIP gene from P. capsici was about 353 amino acids and that of glucanase pcEGase gene from P. colubrinum was about 312 amino acids. GIP gene from P. capsici showed high level of expression at early hours of the inoculation time period and pcEGase gene showed high level of expression at 16 hpi. High level of expression of pcEGase gene at 16 hpi is an indication that the GIP gene is successfully inhibited by the glucanase protein from the plant. Moreover insilico studies gave some hint on the importance of certain sites on the surfaces of both interacting proteins that might be having a role in binding of the two proteins and subsequent reactions thereof. Insilico analysis also conclusively proved that inhibition of glucanase inhibitor protein is mainly caused by recognition of an arginine as well as an isoleucine residue during the interaction of the two proteins.
机译：卵菌的致病菌辣椒疫霉菌（Phytophthora capsici）对黑胡椒（Piper nigrum L.）具有毁灭性，并在植物生长的所有阶段引起脚腐病。疫霉菌分泌一种葡聚糖酶抑制剂蛋白（GIP），该蛋白能够抑制内切葡聚糖酶等防御蛋白。在这项特殊的研究中，定量PCR分析，分子对接研究以及葡聚糖酶抑制剂蛋白和β-1,3葡聚糖基因的序列分析主要是根据辣椒疫霉全基因组测序和Piper colubrinum RNA测序（RNA-序列）。辣椒疫霉的GIP基因的氨基酸序列长度约为353个氨基酸，colubrinum的葡聚糖酶pcEGase基因的氨基酸序列长度约为312个氨基酸。辣椒疫霉的GIP基因在接种时间的早期显示高水平的表达，而pcEGase基因在16hpi时显示高水平的表达。 pcEGase基因在16 hpi的高水平表达表明GIP基因已被植物中的葡聚糖酶蛋白成功抑制。此外，计算机研究表明，两种相互作用蛋白表面上某些位点的重要性可能暗示着这两种蛋白的结合及其后续反应。 Insilico分析还得出结论，葡聚糖酶抑制剂蛋白的抑制主要是由于在两种蛋白相互作用期间识别精氨酸和异亮氨酸残基引起的。

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