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Tissue-Specific Ubiquitination by IPA1 INTERACTING PROTEIN1 Modulates IPA1 Protein Levels to Regulate Plant Architecture in Rice

机译:IPA1相互作用蛋白1的组织特异性泛素化调节IPA1蛋白水平以调节水稻的植物构型

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摘要

Plant architecture, a collection of genetically controlled agronomic traits, is one of the decisive factors that determine grain production. IDEAL PLANT ARCHITECTURE1 (IPA1) encodes a key transcription factor with pleiotropic effects on regulating plant architecture in rice (Oryza sativa), and IPA1 expression is controlled at the posttranscriptional level by microRNA156 and microRNA529. Here, we report the identification and characterization of IPA1 INTERACTING PROTEIN1 (IPI1), a RING-finger E3 ligase that can interact with IPA1 in the nucleus. IPI1 promotes the degradation of IPA1 in panicles, while it stabilizes IPA1 in shoot apexes. Consistent with these findings, the ipi1 loss-of-function mutants showed markedly altered plant architecture, including more tillers, enlarged panicles, and increased yield per plant. Moreover, IPI1 could ubiquitinate the IPA1-mediated complex with different polyubiquitin chains, adding K48-linked polyubiquitin chains in panicles and K63-linked polyubiquitin chains in the shoot apex. These results demonstrate that IPI1 affects plant architecture through precisely tuning IPA1 protein levels in different tissues in rice and provide new insight into the tissue-specific regulation of plant architecture and important genetic resources for molecular breeding.
机译:植物结构是遗传控制的农艺性状的集合,是决定谷物产量的决定性因素之一。理想植物结构1(IPA1)编码对水稻(Oryza sativa)的植物结构有调节作用的关键转录因子,并且microRNA156和microRNA529在转录后水平上控制IPA1的表达。在这里,我们报告鉴定和表征IPA1相互作用蛋白1(IPI1),可以与细胞核中的IPA1相互作用的RING-手指E3连接酶。 IPI1促进圆锥花序中IPA1的降解,同时稳定芽尖中的IPA1。与这些发现一致的是,ipi1功能丧失的突变体显示出植物结构发生了显着变化,包括更多的分till,穗增大和单株产量增加。此外,IPI1可以泛素化IPA1介导的具有不同聚泛素链的复合物,在穗上添加K48连接的聚泛素链,在茎尖添加K63连接的聚泛素链。这些结果表明IPI1通过精确调节水稻不同组织中IPA1的蛋白质水平来影响植物的结构,并为植物结构的组织特异性调节和分子育种的重要遗传资源提供了新的见识。

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