首页> 美国卫生研究院文献>Plant Physiology >Fat Metabolism in Higher Plants XXVI. Biosynthesis of Fatty Acids in Tissues of Developing Seeds and Germinating Seedlings of Safflower (Carthamus tinctorius L.)
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Fat Metabolism in Higher Plants XXVI. Biosynthesis of Fatty Acids in Tissues of Developing Seeds and Germinating Seedlings of Safflower (Carthamus tinctorius L.)

机译:高等植物XXVI中的脂肪代谢。红花(Carthamus tinctorius L.)发育中的种子和发芽幼苗的组织中脂肪酸的生物合成

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摘要

The capacity of both developing seeds and germinating seedlings of safflower for the incorporation of acetate-C14 into long-chain fatty acids is examined.Intact tissue of the developing seed shows a low rate of acetate incorporation into fatty acid initially but between the tenth and twenty-fifth day after flowering the tissue has a high rate of synthesis, in particular with respect to the unsaturated fatty acids. Centrifuged fractionation of homogenates of this developmental tissue yielded several active fractions, the most active being the PI fraction consisting mostly of plastids. Cofactor requirements and pH effects are examined.Germinating tissue shows a more uniform capacity for synthesis of fatty acids since there is no marked change in synthetic capacity. The newly synthesized fatty acids are consistently palmitic, stearic, and oleic acid. No linoleic synthesis could be detected. The most active fraction of cell-free preparation of germinating tissue is the plastid fraction (PI), similar to what was formed with developing tissue.
机译:研究了红花的发芽种子和发芽幼苗将乙酸-C 14 掺入长链脂肪酸的能力。发育中种子的完整组织显示乙酸盐掺入脂肪的比率很低最初是酸,但在开花后的第十至第二十五天之间,组织的合成率很高,特别是对于不饱和脂肪酸而言。该发育组织的匀浆的离心分级分离产生几个活性级分,最活跃的是主要由质体组成的PI级分。检查了辅助因子的要求和pH值的影响。发芽的组织显示出更均匀的脂肪酸合成能力,因为合成能力没有明显变化。新合成的脂肪酸始终是棕榈酸,硬脂酸和油酸。未检测到亚油酸合成。无细胞萌发组织制备中最活跃的部分是质体部分(PI),类似于发育中的组织所形成的部分。

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