首页> 美国卫生研究院文献>Plant Physiology >Effect of Disalicylidenepropanediamine on the Light-dependent Reduction of Carbon Dioxide and Glycerate 3-Phosphate in Intact Spinach Chloroplasts
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Effect of Disalicylidenepropanediamine on the Light-dependent Reduction of Carbon Dioxide and Glycerate 3-Phosphate in Intact Spinach Chloroplasts

机译:二水杨基丙二胺对完整菠菜叶绿体中二氧化碳和3-磷酸甘油酯光依赖性还原的影响

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摘要

Disalicylidenepropanediamine (DSPD) at 0.1 to 1 mm levels inhibited light-dependent 14CO2 assimilation in intact spinach chloroplasts about 50 to 80%, and this inhibition was accompanied by an increased ratio of 14C-glycerate 3-phosphate to 14C-glyceraldehyde 3-phosphate. Enzymatic analysis established that DSPD also inhibited the light-dependent reduction of glycerate 3-phosphate in intact spinach chloroplasts. DSPD at 0.5 mm did not inhibit ribose 5-phosphate isomerase, ribulose 5-phosphate kinase, glycerate 3-phosphate kinase, NADP+-linked glyceraldehyde 3-phosphate dehydrogenase or ribulose 1,5-diphosphate carboxylase. The inhibition of chloroplast 14CO2 assimilation by DSPD appeared to be related to the inhibition of the photosynthetic electron transport chain. These observations are consistent with experimental results which demonstrated that DSPD inhibited directly the chloroplast lamellar membrane-mediated, light-dependent reduction of ferredoxin (Trebst, A. and M. Burba, 1967, Z. Pflanzenphysiol. 57: 419-433 and Ben-Amotz, A. and M. Avron, 1972, Plant Physiol. 49: 244-248).
机译:0.1-1 mm的二水杨基丙二胺(DSPD)抑制完整菠菜叶绿体中依赖光的 14 CO2同化率约50%至80%,并且这种抑制作用伴随着 14 的增加。 sup> C-甘油酸3-磷酸酯生成 14 C-甘油醛3-磷酸酯。酶分析表明,DSPD还抑制了菠菜叶绿体中3-磷酸甘油酯的光依赖性还原。 0.5mm的DSPD不抑制核糖5-磷酸异构酶,核糖5-磷酸激酶,甘油酸3-磷酸激酶,NADP +连接的甘油醛3-磷酸脱氢酶或核糖1,5-二磷酸羧化酶。 DSPD对叶绿体 14 CO2同化的抑制作用可能与抑制光合电子传递链有关。这些观察结果与实验结果一致,实验结果表明DSPD直接抑制叶绿体层状膜介导的铁氧还蛋白的光依赖性还原(Trebst,A. and M. Burba,1967,Z. Pflanzenphysiol。57:419-433 and Ben- Amotz,A。和M.Avron,1972,Plant Physiol.49:244-248)。

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