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A Tight-Seal Whole Cell Study of the Voltage-Dependent Gating Mechanism of K+-Channels of Protoplasmic Droplets of Chara corallina

机译:密闭性炭疽菌原生质滴K +通道电压依赖性门控机制的密闭全细胞研究

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摘要

The biophysical properties of voltage-dependent K+-channels of protoplasmic droplets of Chara corallina Klein ex Willd., em, R.D.W. were investigated using the tight-seal whole cell method. Two potassium currents were observed in voltage-clamp mode and they can be used to explain the transient membrane potential time course observed in current-clamp mode. The K+-channels are identified by the effect of tetraethylammonium chloride which blocks both currents. A two-state, constant dipole moment model is used to fit the voltage-conductance curve. From this model the minimum equivalent gating charge involved in the gating mechanism of K+-channels of Chara can be estimated.
机译:Chara Corallina Klein ex Willd。,em,R.D.W.的原生质滴的质子滴的电压依赖性K + 通道的生物物理特性。使用密闭全细胞方法进行研究。在电压钳位模式下观察到两个钾电流,它们可用于解释在电流钳位模式下观察到的瞬时膜电位时间过程。 K + 通道是通过四乙基氯化铵的作用来识别的,它可以阻断两种电流。使用两态恒定偶极矩模型拟合电压-电导曲线。根据该模型,可以估计参与Chara的K + 通道的门控机制的最小等效门控电荷。

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