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首页> 美国卫生研究院文献>Plant Physiology >7-Dehydrobrefeldin A a naturally occurring brefeldin A derivative inhibits secretion and causes a cis-to-trans breakdown of Golgi stacks in plant cells.
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7-Dehydrobrefeldin A a naturally occurring brefeldin A derivative inhibits secretion and causes a cis-to-trans breakdown of Golgi stacks in plant cells.

机译：7-脱氢布雷菲德菌素A一种天然存在的布雷菲德菌素A衍生物抑制分泌并导致植物细胞中高尔基体的顺式到反式分解。

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7-Dehydrobrefeldin A (7-oxo-BFA) is a brefeldin A (BFA) analog that, like BFA, is a potent phytotoxin of Alternaria carthami, a fungal pathogen of safflower (Carthamus tinctorius L.) plants. Both BFA and 7-oxo-BFA have been shown to be causal agents of the leaf spot disease of these plants. We have investigated the effects of 7-oxo-BFA on the secretion and the structure of the Golgi stacks of sycamore maple (Acer pseudoplatanus) suspension-cultured cells to determine whether 7-oxo-BFA affects these cells in the same manner as BFA. When applied at 10 micrograms/mL for 1 h, 7-oxo-BFA inhibits secretion of proteins by approximately 80%, the same value obtained for BFA. However, electron micrographs of high-pressure frozen/freeze-substituted cells demonstrated that 7-oxo-BFA is a more potent disrupter of the Golgi stacks of sycamore maple cells than BFA. In cells treated for 1 h with 10 micrograms/mL 7-oxo-BFA, very few Golgi stacks can be discerned. Most of those that are left consist of fewer than three cisternae, all of which stain like trans-Golgi cisternae. They are surrounded by clusters of large (150-300 nm in diameter), darkly staining vesicles that are embedded in a fine-filamentous, ribosome-excluding matrix. Similarly sized and stained vesicles are seen budding from the rims of the residual trans-Golgi cisternae. Both the large vesicles and the residual Golgi stack buds stain with anti-xyloglucan polysaccharide antibodies. Recovery of Golgi stacks after removal of 7-oxo-BFA from 1-h-treated cells takes 2 to 6 h, compared with 1 to 2 h for cells treated with BFA. In contrast to 7-oxo-BFA, the BFA breakdown product BFA acid had no effect either on secretion or on the secretory apparatus. This is the first report, to our knowledge of a BFA analog inhibiting secretion in a eukaryotic cell system.
机译：7-脱氢布雷菲德菌素A（7-oxo-BFA）是布雷菲德菌素A（BFA）类似物，与BFA一样，是红花链霉菌（红花（Carthamus tinctorius L.）植物的真菌病原体）的有效植物毒素。已证明BFA和7-氧代BFA都是这些植物的叶斑病的病原体。我们已经研究了7-氧代-BFA对美国梧桐枫（Acer pseudoplatanus）悬浮培养细胞的分泌和高尔基堆栈结构的影响，以确定7-氧代-BFA是否以与BFA相同的方式影响这些细胞。当以10微克/ mL的浓度施用1 h时，7-oxo-BFA抑制蛋白质分泌约80％，与BFA所获得的值相同。但是，高压冷冻/冷冻替代细胞的电子显微照片表明，与BFA相比，7-氧代-BFA是对无花果枫树细胞的高尔基堆栈的更有效的破坏者。在用10微克/ mL 7-oxo-BFA处理1小时的细胞中，几乎看不到高尔基体。剩下的大多数由少于三个的水箱组成，所有这些水箱都像反式高尔基水箱一样染色。它们被簇状（直径150-300 nm）的深色染色囊泡围绕，囊泡包埋在细丝状，不包含核糖体的基质中。从残留的反式高尔基水箱的边缘可以看到大小相似和染色的囊泡。大囊泡和残留的高尔基体芽都用抗木葡聚糖多糖抗体染色。从1小时处理的细胞中去除7-氧代BFA后，恢复高尔基堆栈需要2至6小时，而用BFA处理的细胞则需要1至2小时。与7-氧代-BFA相反，BFA分解产物BFA酸对分泌或对分泌装置没有影响。据我们所知，这是关于BFA类似物抑制真核细胞系统分泌的第一份报告。

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