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首页> 美国卫生研究院文献>Plant Physiology >A Comparative Analysis of the Plant Cellulose Synthase (CesA) Gene Family
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A Comparative Analysis of the Plant Cellulose Synthase (CesA) Gene Family

机译：植物纤维素合成酶（CesA）基因家族的比较分析。

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CesA genes are believed to encode the catalytic subunit of cellulose synthase. Identification of nine distinct CesA cDNAs from maize (Zea mays) has allowed us to initiate comparative studies with homologs from Arabidopsis and other plant species. Mapping studies show that closely related CesA genes are not clustered but are found at different chromosomal locations in both Arabidopsis and maize. Furthermore, sequence comparisons among the CesA-deduced proteins show that these cluster in groups wherein orthologs are often more similar than paralogs, indicating that different subclasses evolved prior to the divergence of the monocot and dicot lineages. Studies using reverse transcriptase polymerase chain reaction with gene-specific primers for six of the nine maize genes indicate that all genes are expressed to at least some level in all of the organs examined. However, when expression patterns for a few selected genes from maize and Arabidopsis were analyzed in more detail, they were found to be expressed in unique cell types engaged in either primary or secondary wall synthesis. These studies also indicate that amino acid sequence comparisons, at least in some cases, may have value for prediction of such patterns of gene expression. Such analyses begin to provide insights useful for future genetic engineering of cellulose deposition, in that identification of close orthologs across species may prove useful for prediction of patterns of gene expression and may also aid in prediction of mutant combinations that may be necessary to generate severe phenotypes.
机译：据信CesA基因编码纤维素合酶的催化亚基。鉴定了来自玉米（Zea mays）的九种不同的CesA cDNA，这使我们能够开始利用拟南芥和其他植物物种的同源物进行比较研究。定位研究表明，紧密相关的CesA基因没有聚簇，但在拟南芥和玉米中的不同染色体位置均发现。此外，CesA推导的蛋白质之间的序列比较显示，这些簇聚在一起，其中直系同源物通常比旁系同源物更相似，这表明不同的亚类在单子叶植物和双子叶植物谱系分化之前进化。使用逆转录酶聚合酶链反应与9个玉米基因中的6个基因的特异性引物进行的研究表明，所有基因在所有受检器官中均至少表达一定水平。然而，当对来自玉米和拟南芥的一些选定基因的表达模式进行更详细的分析时，发现它们在参与初级或次级壁合成的独特细胞类型中表达。这些研究还表明，至少在某些情况下，氨基酸序列比较可能对预测这种基因表达模式具有价值。此类分析开始提供对纤维素沉积的未来基因工程有用的见解，因为跨物种的紧密直向同源物的鉴定可能证明对预测基因表达模式有用，并且还可能有助于预测可能产生严重表型的突变体组合。 。

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