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首页> 美国卫生研究院文献>PLoS Pathogens >Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (Apis mellifera Hymenoptera: Apidae)
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Large-Scale Field Application of RNAi Technology Reducing Israeli Acute Paralysis Virus Disease in Honey Bees (Apis mellifera Hymenoptera: Apidae)

机译：RNAi技术的大规模现场应用减少了蜜蜂中的以色列急性麻痹病毒病（蜜蜂（Apis mellifera膜翅目：Apidae））

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The importance of honey bees to the world economy far surpasses their contribution in terms of honey production; they are responsible for up to 30% of the world's food production through pollination of crops. Since fall 2006, honey bees in the U.S. have faced a serious population decline, due in part to a phenomenon called Colony Collapse Disorder (CCD), which is a disease syndrome that is likely caused by several factors. Data from an initial study in which investigators compared pathogens in honey bees affected by CCD suggested a putative role for Israeli Acute Paralysis Virus, IAPV. This is a single stranded RNA virus with no DNA stage placed taxonomically within the family Dicistroviridae. Although subsequent studies have failed to find IAPV in all CCD diagnosed colonies, IAPV has been shown to cause honey bee mortality. RNA interference technology (RNAi) has been used successfully to silence endogenous insect (including honey bee) genes both by injection and feeding. Moreover, RNAi was shown to prevent bees from succumbing to infection from IAPV under laboratory conditions. In the current study IAPV specific homologous dsRNA was used in the field, under natural beekeeping conditions in order to prevent mortality and improve the overall health of bees infected with IAPV. This controlled study included a total of 160 honey bee hives in two discrete climates, seasons and geographical locations (Florida and Pennsylvania). To our knowledge, this is the first successful large-scale real world use of RNAi for disease control.
机译：蜜蜂对世界经济的重要性远远超过了蜜蜂在蜂蜜生产方面的贡献。他们通过农作物的授粉占世界粮食产量的30％。自2006年秋季以来，美国的蜜蜂种群数量严重下降，部分原因是一种称为“殖民地崩溃症”（CCD）的现象，该现象可能是由多种因素引起的。研究人员比较了受CCD污染的蜜蜂中病原体的初步研究数据表明，以色列急性瘫痪病毒IAPV具有假定作用。这是单链RNA病毒，在分类上没有双链梭菌科的DNA阶段。尽管随后的研究未能在所有CCD确诊的菌落中发现IAPV，但已证明IAPV会导致蜜蜂死亡。 RNA干扰技术（RNAi）已成功用于通过注射和饲喂使内源性昆虫（包括蜜蜂）基因沉默的方法。而且，RNAi被证明可以防止蜜蜂在实验室条件下死于IAPV的感染。在当前的研究中，IAPV特异性同源dsRNA在自然养蜂条件下用于该领域，以防止死亡率和改善被IAPV感染的蜜蜂的整体健康。这项对照研究包括在两个离散的气候，季节和地理位置（佛罗里达州和宾夕法尼亚州）的总共160个蜂巢。据我们所知，这是在现实世界中首次成功地将RNAi用于疾病控制。

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