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Multienzyme Immobilized Polymeric Membrane Reactor for the Transformation of a Lignin Model Compound

机译：固定化多酶聚合物膜反应器转化木质素模型化合物

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摘要

We have developed an integrated, multienzyme functionalized membrane reactor for bioconversion of a lignin model compound involving enzymatic catalysis. The membrane bioreactors were fabricated through the layer-by-layer assembly approach to immobilize three different enzymes (glucose oxidase, peroxidase and laccase) into pH-responsive membranes. This novel membrane reactor couples the in situ generation of hydrogen peroxide (by glucose oxidase) to oxidative conversion of a lignin model compound, guaiacylglycerol-β-guaiacyl ether (GGE). Preliminary investigation of the efficacy of these functional membranes towards GGE degradation is demonstrated under convective flow mode. Over 90% of the initial feed could be degraded with the multienzyme immobilized membranes at a residence time of approximately 22 s. GGE conversion product analysis revealed the formation of oligomeric oxidation products upon reaction with peroxidase, which may be a potential hazard to membrane bioreactors. These oxidation products could further be degraded by laccase enzymes in the multienzymatic membranes, explaining the potential of multi enzyme membrane reactors. The multienzyme incorporated membrane reactors were active for more than 30 days of storage time at 4 °C. During this time span, repetitive use of the membrane reactor was demonstrated involving 5–6 h of operation time for each cycle. The membrane reactor displayed encouraging performance, losing only 12% of its initial activity after multiple cycles of operation.
机译：我们已经开发了一种集成的，多酶功能化的膜反应器，用于涉及酶催化作用的木质素模型化合物的生物转化。膜生物反应器是通过逐层组装的方法制造的，可将三种不同的酶（葡萄糖氧化酶，过氧化物酶和漆酶）固定在pH响应膜中。这种新颖的膜反应器将过氧化氢的原位生成（通过葡萄糖氧化酶）与木质素模型化合物愈创木脂甘油-β-愈创木脂醚（GGE）的氧化转化结合在一起。在对流模式下，对这些功能性膜对GGE降解的功效进行了初步研究。多酶固定膜可在约22 s的停留时间内降解超过90％的初始进料。 GGE转化产物分析表明，与过氧化物酶反应会形成低聚氧化产物，这可能会对膜生物反应器造成潜在危害。这些氧化产物可被多酶膜中的漆酶进一步降解，这说明了多酶膜反应器的潜力。掺有多酶的膜反应器在4°C下可保存30天以上。在这段时间内，证明了膜反应器的重复使用涉及每个循环5-6小时的运行时间。膜反应器表现出令人鼓舞的性能，在多个操作循环后仅损失其初始活性的12％。

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