首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Topology of Repeated Sequences: Relationship of Nuclear RNA to the Repeated Sequences of the Main and Satellite DNA in Mouse Plasmocytoma Cells
【2h】

Topology of Repeated Sequences: Relationship of Nuclear RNA to the Repeated Sequences of the Main and Satellite DNA in Mouse Plasmocytoma Cells

机译:重复序列的拓扑:小鼠浆细胞瘤细胞中核RNA与主要和卫星DNA重复序列的关系

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务,本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文,但由于OA文献来源多样且变更频繁,仍可能出现获取不到、文献不完整或与标题不符等情况,如果获取不到我们将提供退款服务。请知悉。

摘要

The topology of repeated sequences in mouse plasmocytoma DNA was studied by high-resolution CsCl density gradient centrifugation and heterogeneous nuclear RNA·DNA hybridization. Satellite region DNA of plasmocytoma cells contains additional components and hybridizes specifically with entire heterogeneous nuclear RNA molecules. A linkage is demonstrated between the A+T-rich satellite sequences and those hybridizing with heterogeneous nuclear RNA. Heavy DNA also hybridizes specifically with heterogeneous nuclear RNA molecules that show sequence similarity to heterogeneous nuclear RNA hybridized to satellite DNA. These results could suggest that part of satellite DNA became heavier after integration of some other DNA species, which could belong to a virus or to immunoglobulin repetitive genes. Dispersed, highly repetitive, short nucleotide sequences could constitute recognition sites for such a process.
机译:通过高分辨率CsCl密度梯度离心和异质核RNA·DNA杂交研究了小鼠浆细胞瘤DNA重复序列的拓扑。浆细胞瘤细胞的卫星区域DNA包含其他成分,并与整个异质核RNA分子特异性杂交。富含A + T的卫星序列与与异质核RNA杂交的卫星序列之间存在联系。重DNA还与异质核RNA分子特异性杂交,该分子显示出与与卫星DNA杂交的异质核RNA序列相似的序列。这些结果可能表明,卫星DNA的一部分在整合了一些其他DNA物种后变得更重,而其他DNA物种可能属于病毒或免疫球蛋白重复基因。分散的,高度重复的短核苷酸序列可以构成该过程的识别位点。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱:kefu@zhangqiaokeyan.com

京公网安备:11010802029741号 ICP备案号:京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有
  • 客服微信

  • 服务号