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首页> 外文期刊>Gene: An International Journal Focusing on Gene Cloning and Gene Structure and Function >Species-genomic relationships among the tribasic diploid and polyploid Carthamus taxa based on physical mapping of active and inactive 18S-5.8S-26S and 5S ribosomal RNA gene families, and the two tandemly repeated DNA sequences
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Species-genomic relationships among the tribasic diploid and polyploid Carthamus taxa based on physical mapping of active and inactive 18S-5.8S-26S and 5S ribosomal RNA gene families, and the two tandemly repeated DNA sequences

机译：基于有活性和无活性18S-5.8S-26S和5S核糖体RNA基因家族的物理定位以及两个串联重复的DNA序列的三元二倍体和多倍体红花类群之间的物种基因组关系

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摘要

In the genus Carthamus (2n = 20, 22, 24, 44, 64; x = 10, 11, 12), most of the homologues within and between the chromosome complements are difficult to be identified. In the present work, we used fluorescent in situ hybridisation (FISH) to determine the chromosome distribution of the two rRNA gene families, and the two isolated repeated DNA sequences in the 14 Carthamus taxa. The distinctive variability in the distribution, number and signal intensity of hybridisation sites for 18S-26S and 5S rDNA loci could generally distinguish the 14 Carthamus taxa. Active 18S-26S rDNA sites were generally associated with NOR loci on the nucleolar chromosomes. The two A genome taxa, C. glaucus ssp. anatolicus and C. boissieri with 2n = 20, and the two botanical varieties of B genome C. tinctorius (2n = 24) had diagnostic FISH patterns. The present results support the origin of C. tinctorius from C. palaestinus. FISH patterns of C. arborescens vis-à-vis the other taxa indicate a clear division of Carthamus taxa into two distinct lineages. Comparative distribution and intensity pattern of 18S-26S rDNA sites could distinguish each of the tetraploid and hexaploid taxa. The present results indicate that C. boissieri (2n = 20) is one of the genome donors for C. lanatus and C. lanatus ssp. lanatus (2n = 44), and C. lanatus is one of the progenitors for the hexaploid (2n = 64) taxa. The association of pCt. KpnI-2 repeated sequence with rRNA gene cluster (orphon) in 2-10 nucleolar and non-nucleolar chromosomes and the consistent occurrence of pCt. KpnI-1 repeated sequence at the subtelomeric region in all the taxa analysed indicate some functional role of these sequences.
机译：在Carthamus属（2n = 20、22、24、44、64; x = 10、11、12）中，染色体补体之内和之间的大多数同源物很难鉴定。在当前的工作中，我们使用荧光原位杂交（FISH）来确定两个rRNA基因家族的染色体分布，以及14个Carthamus类群中两个分离的重复DNA序列。 18S-26S和5S rDNA基因座的杂交位点的分布，数量和信号强度的明显差异通常可以区分14个红花类群。活性18S-26S rDNA位点通常与核仁染色体上的NOR基因座相关。两个A基因组分类单元，C。glaucus ssp。 anatolicus和C. boissieri的2n = 20，而B基因组C. tinctorius的两个植物变种（2n = 24）具有诊断性FISH模式。目前的结果支持了C. tinctorius起源于C. palaestinus。 C. arborescens相对于其他类群的FISH模式表明将Carthamus类群明确划分为两个不同的谱系。 18S-26S rDNA位点的比较分布和强度模式可以区分四倍体和六倍体类群。目前的结果表明，C。boissieri（2n = 20）是C. lanatus和C. lanatus ssp的基因组供体之一。 lanatus（2n = 44），而C. lanatus是六倍体（2n = 64）类群的祖先之一。 pCt的关联。 KpnI-2在2-10个核仁和非核仁染色体中具有rRNA基因簇（孤儿）的重复序列，并持续出现pCt。在所有分析的分类单元中，亚端粒区域的KpnI-1重复序列表明了这些序列的某些功能。

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