O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

机译：邻苯二甲醛：荧光检测在picomole范围内的伯胺。与荧光胺和茚三酮的比较。

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O-Phthalaldehyde, in the presence of 2-mercaptoethanol, reacts with primary amines to form highly fluorescent products. Picomole quantities of amino acids, peptides, and proteins can be detected easily. o-Phthalaldehyde is five to ten times more sensitive than fluorescamine and is soluble and stable in aqueous buffers.

机译：在2-巯基乙醇的存在下，邻苯二甲醛与伯胺反应形成高度荧光的产物。可以轻松检测出氨基酸，肽和蛋白质的皮摩尔含量。邻苯二甲醛的敏感性比氟胺高五到十倍，并且在水性缓冲液中可溶且稳定。

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
J R Benson; P E Hare;
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年(卷),期 1975(72),2
年度 1975
页码 619–622
总页数 4
原文格式 PDF
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7. O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin. [O] . Benson, J R, Hare, P E 1975

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O-phthalaldehyde: fluorogenic detection of primary amines in the picomole range. Comparison with fluorescamine and ninhydrin.

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