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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Invariant temporal order of replication of the four actin gene loci during the naturally synchronous mitotic cycles of Physarum polycephalum.
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Invariant temporal order of replication of the four actin gene loci during the naturally synchronous mitotic cycles of Physarum polycephalum.

机译：多头Phys头自然同步有丝分裂周期中四个肌动蛋白基因基因座的复制的时间顺序不变。

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摘要

The chronological sequence of replication for the four unlinked actin gene loci of Physarum has been established. Southern hybridization analysis of density-labeled, bromodeoxyuridine-substituted DNA isolated from defined periods of S phase demonstrates that three actin loci (ardB, ardC, ardD) are duplicated early, corresponding to the first 10% of the genome. The fourth locus (ardA) replicates later, between 80 and 100 min into S phase and after 75% of DNA synthesis is completed. Gene-dosage determinations, based on the quantitation of hybridization signals from DNAs isolated from various times during S phase, confirm the results obtained with bromodeoxyuridine-substituted DNA and increase the temporal resolution. The chronological order of replication in the macroplasmodium appears constant through two consecutive cell cycles and after prolonged growth in suspension culture. The precise chronology of DNA synthesis at the gene level extends to the coordinate replication of allele pairs.
机译：已经确定了四个未连接的肌动蛋白肌动蛋白基因基因座的复制时间顺序。从限定的S期分离的密度标记的溴脱氧尿苷取代的DNA的Southern杂交分析表明，三个肌动蛋白基因座（ardB，ardC，ardD）在早期复制，对应于基因组的前10％。第四个基因座（ardA）随后复制，进入S期80至100分钟，DNA合成完成75％之后。基于对从S期不同时间分离的DNA杂交信号进行定量的基因剂量确定，可以确认使用溴脱氧尿苷取代的DNA所获得的结果，并提高了时间分辨率。在两个连续的细胞周期中以及在悬浮培养物中长时间生长后，大质粒中复制的时间顺序似乎是恒定的。基因水平上DNA合成的精确时间顺序扩展到等位基因对的坐标复制。

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