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首页> 外文期刊>Cell motility and the cytoskeleton >Regulation of levels of actin threonine phosphorylation during life cycle of Physarum polycephalum.
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Regulation of levels of actin threonine phosphorylation during life cycle of Physarum polycephalum.

机译：多头Phys草生命周期中肌动蛋白苏氨酸磷酸化水平的调节。

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摘要

Under various environmental stresses, the true slime mold Physarum polycephalum converts into dormant forms, such as microcysts, sclerotia, and spores, which can survive in adverse environments for a considerable period of time. In drought-induced sclerotia, actin is threonine phosphorylated, which blocks its ability to polymerize into filaments. It is known that fragmin and actin-fragmin kinase (AFK) mediate this phosphorylation event. In this work, we demonstrate that high levels of actin threonine phosphorylation are also found in other dormant cells, including microcysts and spores. As the threonine phosphorylation of actin in microcysts and sclerotia were induced by drought stress but not by other stresses, we suggest that drought stress is essential for actin phosphorylation in both cell types. Although characteristic filamentous actin structures (dot- or rod-like structures) were observed in microcysts, sclerotia, and spores, actin phosphorylation was not required for the formation of these structures. Prior to the formation of both microcysts and sclerotia, AFK mRNA expression was activated transiently, whereas fragmin mRNA levels decreased. Our results suggest that drought stress and AFK might be involved in the threonine phosphorylation of actin.
机译：在各种环境压力下，真正的粘液霉菌多头Phys麦草会转变成休眠形式，例如微囊，菌核和孢子，它们可以在不利的环境中存活相当长的一段时间。在干旱引起的菌核中，肌动蛋白被苏氨酸磷酸化，从而阻止其聚合成细丝的能力。众所周知，fragmin和肌动蛋白-fragmin激酶（AFK）介导此磷酸化事件。在这项工作中，我们证明在其他休眠细胞（包括微囊和孢子）中也发现了高水平的肌动蛋白苏氨酸磷酸化。由于微囊藻和菌核中肌动蛋白的苏氨酸磷酸化是由干旱胁迫而非其他胁迫诱导的，因此我们建议干旱胁迫对于两种细胞类型的肌动蛋白磷酸化都是必不可少的。尽管在微囊肿，菌核和孢子中观察到特征性的丝状肌动蛋白结构（点状或棒状结构），但形成这些结构不需要肌动蛋白磷酸化。在微囊和菌核形成之前，AFK mRNA表达被瞬时激活，而fragmin mRNA水平降低。我们的结果表明干旱胁迫和AFK可能与肌动蛋白的苏氨酸磷酸化有关。

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