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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Isolation of infectious human T-cell leukemia/lymphotropic virus type III (HTLV-III) from patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) and from healthy carriers: a study of risk groups and tissue sources.
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Isolation of infectious human T-cell leukemia/lymphotropic virus type III (HTLV-III) from patients with acquired immunodeficiency syndrome (AIDS) or AIDS-related complex (ARC) and from healthy carriers: a study of risk groups and tissue sources.

机译：从获得性免疫缺陷综合征（AIDS）或AIDS相关综合症（ARC）患者和健康携带者中分离感染性人类T细胞白血病/ III型淋巴病毒（HTLV-III）：对危险人群和组织来源的研究。

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Acquired immunodeficiency syndrome (AIDS) and AIDS-related complex (ARC) are thought to be caused by human T-cell leukemia/lymphotropic virus type III (HTLV-III). Since the fall of 1982, independent isolates of HTLV-III have been obtained in this laboratory, in collaboration with several clinical groups, from 101 AIDS and ARC patients and healthy donors at risk for AIDS. Most isolates were from peripheral blood T lymphocytes established in cell culture, but some were obtained from bone marrow, lymph node, brain tissue, and cell-free plasma and from cells associated with saliva, cerebrospinal fluid, and semen. Virus was isolated from approximately 50% of AIDS patients, 85% of ARC patients, and 30% of healthy individuals at risk for AIDS. The risk groups included homosexuals, promiscuous heterosexuals, i.v. drug users, recipients of blood or blood products, and spouses and offspring of AIDS patients and others at risk for AIDS. A high correlation was seen between persistent levels of serum antibody and the ability to isolate virus from patient or donor leukocytes. Immunologic and nucleic acid analysis demonstrated that the virus isolates were highly related, although substantial diversity was observed in the restriction enzyme cleavage patterns of those studied in detail. Biological analysis of cells from infected patients and donors as well as from normal peripheral blood mononuclear cells exposed to virus in vitro demonstrated that OKT4/Leu3a+ (helper/inducer) lymphocytes were preferentially infected and were subjected to a characteristic cytopathic effect. The availability of multiple isolates of virus from a number of different patients and donors will greatly facilitate the characterization of HTLV-III and the study of possible biological and/or biochemical variants of the virus responsible for the development of AIDS, ARC, and related diseases.
机译：人们认为后天免疫机能丧失综合症（AIDS）和艾滋病相关综合症（ARC）是由人类T细胞白血病/淋巴病毒III型（HTLV-III）引起的。自1982年秋季以来，在这个实验室中，与101个AIDS和ARC患者以及处于艾滋病风险中的健康捐献者的几个临床小组合作，已经获得了独立的HTLV-III分离株。大多数分离物来自细胞培养物中建立的外周血T淋巴细胞，但有些分离物则来自骨髓，淋巴结，脑组织和无细胞血浆以及与唾液，脑脊髓液和精液有关的细胞。从大约50％的AIDS患者，85％的ARC患者和30％的具有AIDS风险的健康个体中分离出病毒。危险人群包括同性恋，滥交异性恋者。吸毒者，血液或血液制品的接受者，以及艾滋病患者和其他有艾滋病风险的人的配偶和后代。血清抗体的持续水平与从患者或供体白细胞分离病毒的能力之间存在高度相关性。免疫学和核酸分析表明病毒分离物高度相关，尽管在详细研究的限制酶切割模式中观察到了实质性的多样性。对来自受感染患者和供体的细胞以及体外暴露于病毒的正常外周血单个核细胞的细胞进行的生物学分析表明，OKT4 / Leu3a +（辅助细胞/诱导细胞）淋巴细胞被优先感染并具有特征性的细胞病变作用。来自许多不同患者和供体的多种病毒分离株的可用性将极大地促进HTLV-III的表征以及研究可能导致AIDS，ARC和相关疾病发展的病毒可能的生物学和/或生化变体。

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