Tobacco chloroplast tRNALys(UUU) gene contains a 2.5-kilobase-pair intron: An open reading frame and a conserved boundary sequence in the intron

机译：烟草叶绿体tRNALys（UUU）基因包含一个2.5碱基对的内含子：一个开放阅读框和一个内含子保守的边界序列

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摘要

The nucleotide sequence of a tRNA^Lys(UUU) gene on tobacco (Nicotiana tabacum) chloroplast DNA has been determined. This gene is located 215 base pairs upstream from the gene for the 32,000-dalton thylakoid membrane protein on the same DNA strand and has a 2526-base-pair intron in the anticodon loop. The intron boundary sequence does not follow the G-U/A-G rule but is similar to those of tobacco chloroplast split genes for tRNA^Gly(UCC) and ribosomal proteins L2 and S12. The intron contains one major open reading frame of 509 codons. The codon usage in the open reading frame resembles those observed in the genes for tobacco chloroplast proteins so far analyzed. The primary transcript of this tRNA gene is 2.7 kilobases long.

机译：确定了烟草（Nicotiana tabacum）叶绿体DNA上的tRNA ^{Lys （UUU）基因的核苷酸序列。该基因位于同一DNA链上32,000道尔顿类囊体膜蛋白的基因上游215个碱基对，并且在反密码子环中具有2526个碱基对的内含子。内含子边界序列不遵循G-U / A-G规则，但与tRNA ^{Gly （UCC）和核糖体蛋白L2和S12的烟草叶绿体分裂基因相似。内含子包含一个主要的509个密码子的开放阅读框。开放阅读框中的密码子用法类似于迄今为止分析的烟草叶绿体蛋白基因中观察到的密码子用法。该tRNA基因的主要转录本长2.7 KB。}}

著录项

期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
Mamoru Sugita; Kazuo Shinozaki; Masahiro Sugiura;
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年(卷),期 1985(82),11
年度 1985
页码 3557–3561
总页数 5
原文格式 PDF
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专利

1. Targeted Inactivation of a Tobacco Intron–containing Open Reading Frame Reveals a Novel Chloroplast-encoded Photosystem I–related Gene [J] . Stephanie Ruf, Hans K?ssel, Ralph Bock Journal of cell biology . 1997,第1期

机译：烟草内含子的开放阅读框的靶向失活揭示了一种新型的叶绿体编码的光系统I相关基因
2. The mitochondrial genome of Morchella importuna (272.2 kb) is the largest among fungi and contains numerous introns, mitochondrial non-conserved open reading frames and repetitive sequences [J] . Liu Wei, Cai Yingli, Zhang Qianqian, International Journal of Biological Macromolecules: Structure, Function and Interactions . 2020,第期

机译：Morchella Importuna（272.2 KB）的线粒体基因组是真菌中最大的，含有许多内含子，线粒体非保守开放阅读框架和重复序列
3. Evidence for a group II intron in Escherichia coli inserted into a highly conserved reading frame associated with mobile DNA sequences [J] . Axel Brennicke, Volker Knoop Nucleic acids research . 1994,第7期

机译：将大肠杆菌中的II组内含子插入与移动DNA序列相关的高度保守的阅读框中的证据
4. Detection of tRNA genes with introns from DNA sequences of archaea [C] . Makoto Kinouchi, Yoshihiro Kudo Workshop on Genome Informatics . 2001

机译：检测古代DNA序列与内含子的TRNA基因
5. Group I intron mediated trans-splicing of highly conserved dengue virus sequences & a mutational analysis of the piggyBac transposase [D] . Keith, James H. 2008

机译：第一组由内含子介导的高度保守的登革热病毒序列的剪接和piggyBac转座酶的突变分析
6. Targeted Inactivation of a Tobacco Intron–containing Open Reading Frame Reveals a Novel Chloroplast-encoded Photosystem I–related Gene [O] . Stephanie Ruf, Hans Kössel, Ralph Bock 1997

机译：烟草内含子的开放阅读框的靶向失活揭示了一种新型的叶绿体编码的光系统I相关基因
7. Tobacco chloroplast tRNALys(UUU) gene contains a 2.5-kilobase-pair intron: An open reading frame and a conserved boundary sequence in the intron [O] . Sugita, Mamoru, Shinozaki, Kazuo, Sugiura, Masahiro 1985

机译：烟草叶绿体tRNALys（UUU）基因包含一个2.5碱基对的内含子：一个开放阅读框和一个内含子保守的边界序列
8. Processing of Archaebacterial Intron-Containing tRNA Gene Transcripts [R] . Daniels, C. J. 1989

机译：处理含有古细菌内含子的tRNa基因转录本

Tobacco chloroplast tRNALys(UUU) gene contains a 2.5-kilobase-pair intron: An open reading frame and a conserved boundary sequence in the intron

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