Crosslinking of hemin to a specific site on the 90-kDa ferritin repressor protein.

机译：血红素交联到90 kDa铁蛋白阻遏蛋白上的特定位点。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

Incubation of a 90-kDa ferritin repressor protein (FRP) with small amounts of radiolabeled hemin resulted in the formation of a strong interaction between the two that was stable to SDS/PAGE. (We refer to this interaction as a "crosslink," without intending to imply knowledge as to its chemical nature.) Of seven other proteins tested individually, only apohemopexin and bovine serum albumin showed similar crosslinking ability, albeit to a much lower extent. [14C]Hemin specifically crosslinked to FRP in the presence of a 50-fold excess of total wheat germ proteins. Inclusion of catalase did not prevent the reaction of hemin with FRP, suggesting that H2O2 is not involved. The subsequent addition of a stoichiometric amount of apohemopexin did not reverse the reaction. Exhaustive digestion of the complex with Staphylococcus aureus V8 protease produced a major labeled peptide of 17 kDa. These results show the existence of a highly specific, uniquely reactive hemin binding site on FRP.

机译：将90 kDa铁蛋白阻遏蛋白（FRP）与少量放射性标记的血红素一起孵育会导致两者之间形成对SDS / PAGE稳定的强相互作用。（我们将这种相互作用称为“交联”，无意暗示对其化学性质的了解。）在单独测试的其他七个蛋白中，只有载脂蛋白新蛋白和牛血清白蛋白显示出相似的交联能力，尽管程度要低得多。 [14C] Hemin在小麦胚芽总蛋白过量50倍的情况下特异性交联至FRP。包含过氧化氢酶并不能阻止血红素与FRP的反应，这表明不涉及H2O2。随后添加化学计量的脱辅酶血红蛋白没有使反应逆转。用金黄色葡萄球菌V8蛋白酶对复合物进行详尽的消化，产生了一个主要的17 kDa标记肽。这些结果表明在FRP上存在高度特异性的，独特的反应性血红素结合位点。

著录项

期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
J J Lin; M M Patino; L Gaffield; W E Walden; A Smith; R E Thach;
展开▼
作者单位

展开▼
年(卷),期 1991(88),14
年度 1991
页码 6068–6071
总页数 4
原文格式 PDF
正文语种
中图分类
关键词

相似文献

外文文献
中文文献
专利

1. Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin [J] . Seki T, Kunichika T, Watanabe K, BioMetals: An International Journal on the Role of Metal Ions in Biology, Biochemistry and Medicine . 2008,第1期

机译：载脂蛋白B通过血红素介导的结合结合铁蛋白：载脂蛋白B和铁蛋白与血红素直接结合的证据
2. Apolipoprotein B binds ferritin by hemin-mediated binding: evidence of direct binding of apolipoprotein B and ferritin to hemin [J] . Takenori Seki, Tomoya Kunichika, Kiyotaka Watanabe, BioMetals . 2008,第1期

机译：载脂蛋白B通过血红素介导的结合结合铁蛋白：载脂蛋白B和铁蛋白与血红素直接结合的证据
3. Hemin uptake in Porphyromonas gingivalis: Omp26 is a hemin-binding surface protein. [J] . T E Bramanti, S C Holt Journal of bacteriology . 1993,第22期

机译：牙龈卟啉单胞菌中的血红素摄取：Omp26是与血红素结合的表面蛋白。
4. Biologically Derived Nanoparticle Arrays via a Site-Specific Reconstitu-tion of Ferritin and Their Electrochemistry [C] . Jae-Woo Kim, Sang H. Choi, Peter T. Lillehei, International Symposium on Nanoscale Devices, Materials, and Biological Systems: Fundamentals and Applications . 2005

机译：通过特异性重构的铁蛋白及其电化学来生物衍生的纳米颗粒阵列
5. Metal catalyzed tyrosine-tyrosine crosslinking: Progress toward the site-specific, oriented immobilization of proteins to surfaces. [D] . Stayner, Richard Scott. 2007

机译：金属催化的酪氨酸-酪氨酸交联：朝着蛋白质在表面上定向固定化的方向发展。
6. Specificity of the protein kinase activity associated with the hemin-controlled repressor of rabbit reticulocyte. [O] . G Kramer, J M Cimadevilla, B Hardesty 1976

机译：与兔网织红细胞血红素控制的阻遏物相关的蛋白激酶活性的特异性。
7. Crosslinking of hemin to a specific site on the 90-kDa ferritin repressor protein. [O] . Lin, J J, Patino, M M, Gaffield, L, 1991

机译：血红素交联到90 kDa铁蛋白阻遏蛋白上的特定位点。

Crosslinking of hemin to a specific site on the 90-kDa ferritin repressor protein.

摘要

著录项

相似文献

相关主题

期刊订阅