Both purified human 1N6-ethenoadenine-binding protein and purified human 3-methyladenine-DNA glycosylase act on 1N6-ethenoadenine and 3-methyladenine.

机译：纯化的人1N6-乙腺嘌呤结合蛋白和纯化的人3-甲基腺嘌呤-DNA糖基化酶均作用于1N6-乙腺嘌呤和3-甲基腺嘌呤。

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摘要

We previously described a protein, isolated from human tissues and cells, that bound to a defined double-stranded oligonucleotide containing a single site-specifically placed 1,N6-ethenoadenine. It was further demonstrated that this protein was a glycosylase and released 1,N6-ethenoadenine. We now find that this enzyme also releases 3-methyladenine from methylated DNA and that 3-methyladenine-DNA glycosylase behaves in the same manner, binding to the ethenoadenine-containing oligonucleotide and cleaving both ethenoadenine and 3-methyladenine from DNA containing these adducts. The rate and extent of glycosylase activities toward the two adducts are similar.

机译：我们先前描述了一种从人体组织和细胞中分离的蛋白质，该蛋白质与定义的双链寡核苷酸结合，该双链寡核苷酸包含一个特定位点的1，N6-乙炔腺嘌呤。进一步证明该蛋白是糖基化酶并释放出1，N6-乙炔腺嘌呤。现在我们发现，该酶还从甲基化的DNA中释放出3-甲基腺嘌呤，并且3-甲基腺嘌呤-DNA糖基化酶的行为相同，与含乙炔腺嘌呤的寡核苷酸结合，并从包含这些加合物的DNA中裂解出乙腺嘌呤和3-甲基腺嘌呤。糖基化酶对两个加合物的活性的速率和程度是相似的。

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
B Singer; A Antoccia; A K Basu; M K Dosanjh; H Fraenkel-Conrat; P E Gallagher; J T Kuśmierek; Z H Qiu; B Rydberg;
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年(卷),期 1992(89),20
年度 1992
页码 9386–9390
总页数 5
原文格式 PDF
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1. Lymphocyte-dependent inhibition of growth of virulent Mycobacterium tuberculosis H37Rv within human monocytes: requirement for CD4+ T cells in purified protein derivative-positive, but not in purified protein derivative-negative subjects. [J] . Silver RF, Li Q, Boom WH, The Journal of Immunology: Official Journal of the American Association of Immunologists . 1998,第5期

机译：人单核细胞内强力结核分枝杆菌H37Rv的淋巴细胞依赖性抑制作用：纯化蛋白衍生物阳性的受试者对CD4 + T细胞的需求，但对纯化蛋白衍生物阴性的受试者却没有要求。
2. Lymphocyte-Dependent Inhibition of Growth of Virulent Mycobacterium tuberculosis H37Rv Within Human Monocytes: Requirement for CD4+ T Cells in Purified Protein Derivative-Positive, But Not in Purified Protein Derivative-Negative Subjects [J] . Richard F. Silver, Qing Li, W. Henry Boom, The journal of immunology . 1998,第5期

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3. Immuno-Precipitates of Some Purified and Unpurified Human Plasma Proteins with Monospecific and Polyvalent Human Plasma Protein Antisera [J] . M. Ceska The journal of immunology . 1969,第1期

机译：具有单特异性和多价人血浆蛋白抗血清的某些纯化和未纯化的人血浆蛋白的免疫沉淀
4. Rapid and sensitive gel-free approach to dissect affinity purified human protein complexes by nanoLC-MSMS [C] . Junqueira M, Toyoda Y, Slabicki M, ASMS Conference on Mass Spectrometry and Allied Topics . 2007

机译：快速敏感的无凝胶方法通过Nanolc-MSMS对亲和纯化的人蛋白复合物进行分离
5. The effects of a purified B-group soyasaponin fraction on the proliferation, cell cycle progression and induction of macroautophagy in HCT-15 human colon adenocarcinoma cells. [D] . Ellington, Allison Ann. 2005

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6. Absence of cytotoxic antibody to human immunodeficiency virus-infected cells in humans and its induction in animals after infection or immunization with purified envelope glycoprotein gp120. [O] . P L Nara, W G Robey, M A Gonda, 2017

机译：用纯化的包膜糖蛋白gp120感染或免疫后，在人体内不存在针对人类免疫缺陷病毒感染的细胞的细胞毒性抗体及其在动物中的诱导。
7. Both purified human 1,N6-ethenoadenine-binding protein and purified human 3-methyladenine-DNA glycosylase act on 1,N6-ethenoadenine and 3-methyladenine. [O] . Singer, B, Antoccia, A, Basu, A K, 1992

机译：纯化的人1，N6-乙腺嘌呤结合蛋白和纯化的人3-甲基腺嘌呤-DNA糖基化酶均作用于1，N6-乙腺嘌呤和3-甲基腺嘌呤。

Both purified human 1N6-ethenoadenine-binding protein and purified human 3-methyladenine-DNA glycosylase act on 1N6-ethenoadenine and 3-methyladenine.

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