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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae.
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Genes encoding transcription factor IIIA and the RNA polymerase common subunit RPB6 are divergently transcribed in Saccharomyces cerevisiae.

机译：编码转录因子IIIA和RNA聚合酶共有亚基RPB6的基因在酿酒酵母中被不同地转录。

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摘要

The gene encoding Saccharomyces cerevisiae transcription factor TFIIIA has been found adjacent to RPB6, a gene that specifies a subunit shared by nuclear RNA polymerases. Analysis of DNA upstream of the RPB6 gene revealed an open reading frame that predicts a protein, designated PZF1, with nine C2H2 zinc fingers. The presence of nine C2H2 zinc fingers in PZF1 protein, a hallmark of amphibian TFIIIA proteins, suggested that PZF1 might be a TFIIIA homologue. We found that purified recombinant PZF1 specifically binds the internal control region (ICR) of the 5S rRNA gene in S. cerevisiae. The presence of nine C2H2 zinc fingers, the specific binding to ICR DNA, and the similarity of the predicted molecular mass of PZF1 with that determined for purified yeast TFIIIA, together indicate that PZF1 is TFIIIA. The yeast and amphibian TFIIIA proteins share only a limited number of residues outside of those normally conserved in C2H2 zinc fingers; these conserved residues may provide clues to the sequence specificity of these proteins. The PZF1 gene was found to be single copy, transcribed into a 1.5-kilobase mRNA, and essential for yeast cell viability. Interestingly, the yeast RPB6 and TFIIIA coding sequences are divergently transcribed and are separated by only 233 base pairs, providing the potential for coregulated expression of components of RNA polymerases and the 5S rRNA component of ribosomes.
机译：已经发现编码酿酒酵母转录因子TFIIIA的基因与RPB6相邻，该基因指定了核RNA聚合酶共有的一个亚基。对RPB6基因上游DNA的分析揭示了一个开放阅读框，该框预测一个名为PZF1的蛋白质，具有9个C2H2锌指。两栖动物TFIIIA蛋白的标志PZF1蛋白中存在9个C2H2锌指，这表明PZF1可能是TFIIIA同源物。我们发现纯化的重组PZF1特异性结合酿酒酵母中5S rRNA基因的内部控制区（ICR）。九个C2H2锌指的存在，与ICR DNA的特异性结合以及PZF1的预测分子量与纯化酵母TFIIIA的分子量相似，共同表明PZF1是TFIIIA。酵母和两栖类TFIIIA蛋白在C2H2锌指中通常保守的残基之外仅共享有限数量的残基。这些保守的残基可能为这些蛋白质的序列特异性提供线索。发现PZF1基因是单拷贝的，转录为1.5碱基碱基的mRNA，对于酵母细胞的生存力至关重要。有趣的是，酵母RPB6和TFIIIA编码序列是不同转录的，仅被233个碱基对隔开，为RNA聚合酶的成分和核糖体的5S rRNA成分的共表达提供了潜力。

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