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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Bidirectional uncoating of the genomic RNA of a helical virus.
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Bidirectional uncoating of the genomic RNA of a helical virus.

机译：螺旋病毒基因组RNA的双向脱膜。

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摘要

An essential step in the initiation of a virus infection is the release of the viral genome from the other constituents of the virus particle, a process referred to as uncoating. We have used reverse transcription and polymerase chain reaction amplification procedures to determine the rate and direction of in vivo uncoating of the rod-shaped tobacco mosaic virus. The virus particles contain a single 6.4-kb RNA molecule that lies between successive turns of a helical arrangement of coat protein subunits. When the particles are introduced into plant cells, the subunits are removed via a bidirectional uncoating mechanism. Within 2-3 min, the part of the viral RNA from the 5' end to a position >70% toward the 3' end has been freed of coat protein subunits. This is followed by removal of subunits from the 3' end of the RNA and sequential uncoating of the RNA in a 3'-to-5' direction. An internal region of the viral RNA is the final part to be uncoated. Progeny virus particles are detected in the cells 35-40 min after inoculation.
机译：引发病毒感染的关键步骤是从病毒颗粒的其他成分释放病毒基因组，这一过程称为脱壳。我们已经使用逆转录和聚合酶链反应扩增程序来确定杆状烟草花叶病毒在体内脱壳的速率和方向。病毒颗粒包含单个6.4 KB RNA分子，该分子位于外壳蛋白亚基螺旋排列的连续序列之间。当将颗粒引入植物细胞后，亚基通过双向脱膜机制被去除。在2-3分钟内，从5'端到3'端> 70％的位置的病毒RNA部分已被去除外壳蛋白亚基。随后是从RNA的3'末端去除亚基，并按3'到5'的方向依次解开RNA。病毒RNA的内部区域是未包被的最后部分。接种后35-40分钟，在细胞中检测到子代病毒颗粒。

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