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Bidirectional uncoating of the genomic RNA of a helical virus

机译:螺旋病毒基因组RNA的双向脱膜

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摘要

An essential step in the initiation of a virus infection is the release of the viral genome from the other constituents of the virus particle, a process referred to as uncoating. We have used reverse transcription and polymer- ase chain reaction amplification procedures to determine the rate and direction of in vivo uncoating of the rod-shaped tobacco mosaic virus. The virus particles contain a single 6.4-kb RNA molecule that lies between successive turns of a helical arrangement of coat protein subunits.
机译:引发病毒感染的关键步骤是从病毒颗粒的其他成分释放病毒基因组,这一过程称为脱壳。我们已经使用逆转录和聚合酶链反应扩增程序来确定杆状烟草花叶病毒在体内脱膜的速率和方向。病毒颗粒包含单个6.4 KB RNA分子,该分子位于外壳蛋白亚基螺旋排列的连续序列之间。

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