• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Transduction of pluripotent human hematopoietic stem cells demonstrated by clonal analysis after engraftment in immune-deficient mice.
【2h】

Transduction of pluripotent human hematopoietic stem cells demonstrated by clonal analysis after engraftment in immune-deficient mice.

机译：在免疫缺陷小鼠中植入后通过克隆分析证明了多能人类造血干细胞的转导。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Gene transduction of pluripotent human hematopoietic stem cells (HSCs) is necessary for successful gene therapy of genetic disorders involving hematolymphoid cells. Evidence for transduction of pluripotent HSCs can be deduced from the demonstration of a retroviral vector integrated into the same cellular chromosomal DNA site in myeloid and lymphoid cells descended from a common HSC precursor. CD34+ progenitors from human bone marrow and mobilized peripheral blood were transduced by retroviral vectors and used for long-term engraftment in immune-deficient (beigeude/XIS) mice. Human lymphoid and myeloid populations were recovered from the marrow of the mice after 7-11 months, and individual human granulocyte-macrophage and T-cell clones were isolated and expanded ex vivo. Inverse PCR from the retroviral long terminal repeat into the flanking genomic DNA was performed on each sorted cell population. The recovered cellular DNA segments that flanked proviral integrants were sequenced to confirm identity. Three mice were found (of 24 informative mice) to contain human lymphoid and myeloid populations with identical proviral integration sites, confirming that pluripotent human HSCs had been transduced.
机译：多能性人类造血干细胞（HSC）的基因转导对于涉及血淋巴样细胞的遗传疾病的成功基因治疗是必要的。多能性HSC的转导证据可以通过将逆转录病毒载体整合到同一HSC前体的髓样和淋巴样细胞的同一细胞染色体DNA位点中来证明。通过逆转录病毒载体转导人骨髓和动员的外周血中的CD34 +祖细胞，并用于免疫缺陷（beige / nude / XIS）小鼠的长期移植。在7-11个月后，从小鼠的骨髓中回收了人类淋巴和髓样群体，并分离了单个人类粒细胞巨噬细胞和T细胞克隆，并进行了离体扩增。从逆转录病毒长末端重复序列到侧翼基因组DNA的反向PCR在每个分选的细胞群上进行。对位于原病毒整合体侧翼的回收细胞DNA片段进行测序，以确认身份。发现三只小鼠（24只信息丰富的小鼠）中含有具有相同前病毒整合位点的人淋巴和髓样群体，这证实了多能人HSC已被转导。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号