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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Lune/eye gone a Pax-like protein uses a partial paired domain and a homeodomain for DNA recognition
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Lune/eye gone a Pax-like protein uses a partial paired domain and a homeodomain for DNA recognition

机译：Lune / eye走了一种Pax样蛋白使用部分配对结构域和同源结构域进行DNA识别

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摘要

Pax proteins, characterized by the presence of a paired domain, play key regulatory roles during development. The paired domain is a bipartite DNA-binding domain that contains two helix–turn–helix domains joined by a linker region. Each of the subdomains, the PAI and RED domains, has been shown to be a distinct DNA-binding domain. The PAI domain is the most critical, but in specific circumstances, the RED domain is involved in DNA recognition. We describe a Pax protein, originally called Lune, that is the product of the Drosophila eye gone gene (eyg). It is unique among Pax proteins, because it contains only the RED domain. eyg seems to play a role both in the organogenesis of the salivary gland during embryogenesis and in the development of the eye. A high-affinity binding site for the Eyg RED domain was identified by using systematic evolution of ligands by exponential enrichment techniques. This binding site is related to a binding site previously identified for the RED domain of the Pax-6 5a isoform. Eyg also contains another DNA-binding domain, a Prd-class homeodomain (HD), whose palindromic binding site is similar to other Prd-class HDs. The ability of Pax proteins to use the PAI, RED, and HD, or combinations thereof, may be one mechanism that allows them to be used at different stages of development to regulate various developmental processes through the activation of specific target genes.
机译：以成对结构域的存在为特征的Pax蛋白在发育过程中起着关键的调控作用。配对结构域是一个二聚体DNA结合结构域，包含两个由接头区域连接的螺旋-转-螺旋结构域。已显示每个子域（PAI和RED域）是不同的DNA结合域。 PAI域是最关键的，但在特定情况下，RED域参与DNA识别。我们描述了一种Pax蛋白，最初称为Lune，是果蝇无视基因（eyg）的产物。它在Pax蛋白中是唯一的，因为它仅包含RED域。 eyg似乎在胚胎发生过程中唾液腺的器官发生和眼睛发育中都起作用。通过利用指数富集技术对配体进行系统进化，确定了Eyg RED域的高亲和力结合位点。该结合位点与先前针对Pax-6 5a同工型的RED结构域鉴定的结合位点有关。 Eyg还包含另一个DNA结合域，即Prd类同源域（HD），其回文结合位点类似于其他Prd类HD。 Pax蛋白使用PAI，RED和HD或其组合的能力可能是一种机制，可以使它们在不同的发育阶段使用，以通过激活特定靶基因来调节各种发育过程。

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