An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels

机译：细胞内磷酸盐缓冲液可过滤细胞外磷酸盐水平的瞬时波动

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To survive in a dynamic and unpredictable environment, cells must correctly interpret and integrate extracellular signals with internal factors. In particular, internal stores of nutrients must be managed for use during periods of nutrient limitation. To gain insight into this complex process, we combined biochemical and spectroscopic techniques to follow the dynamics of the phosphate responsive signaling pathway in both single yeast cells and populations. We demonstrate that the phosphate-responsive genes PHO5 and PHO84 exhibit different kinetics of transcriptional induction in response to phosphate starvation, and that transient phosphate limitation causes induction of PHO84 but not PHO5. This differential kinetic behavior is largely eliminated in cells that lack the ability to store phosphate internally in the form of polyphosphate, but the threshold of external phosphate required for induction of PHO5 and PHO84 is unaffected. Our observations indicate that polyphosphate acts as a buffer that can be mobilized during periods of phosphate limitation and enables the phosphate-responsive signaling pathway to filter transient fluctuations in extracellular phosphate levels.

机译：为了在动态，不可预测的环境中生存，细胞必须正确地解释和整合细胞外信号和内部因素。特别是，必须管理营养素的内部存储以在营养素限制期间使用。为了深入了解这个复杂的过程，我们结合了生物化学和光谱技术，以追踪单个酵母细胞和群体中磷酸盐反应性信号通路的动态。我们证明磷酸盐响应基因PHO5和PHO84表现出不同的响应磷饥饿的转录诱导动力学，并且短暂的磷酸盐限制导致PHO84的诱导而不是PHO5的诱导。在缺乏以聚磷酸盐形式内部存储磷酸盐的能力的细胞中，这种差异动力学行为被大大消除，但是诱导PHO5和PHO84所需的外部磷酸盐阈值不受影响。我们的观察结果表明，多磷酸盐可作为一种缓冲剂，可以在磷酸盐限制期间动员起来，并使磷酸盐反应性信号通路能够过滤细胞外磷酸盐水平的瞬时波动。

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期刊名称 Proceedings of the National Academy of Sciences of the United States of America
作者
Melissa R. Thomas; Erin K. OShea;
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年(卷),期 2005(102),27
年度 2005
页码 9565–9570
总页数 6
原文格式 PDF
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关键词
homeostasis nutrients polyphosphate signaling;

机译：稳态;营养;多磷酸盐;信号;

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3. An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels [J] . Thomas MR, OShea EK Proceedings of the National Academy of Sciences of the United States of America . 2005,第27期

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4. Milli Gauss Pulsed Magnetic Field Applied Phosphate Buffered Saline Solution Elevates Intracellular Ca~(2+) Level and Stimulates Phagocytic Activity of Human Neutrophils [J] . Masanori Fukushima, Kaneo Mohri, Takuji Kataoka Transactions of the Magnetics Society of Japan . 2002,第2Suppla期

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9. An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels [O] . Thomas, Melissa R., O'Shea, Erin K. 2005

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10. Test Protocols & Data Summaries for T-butyl Phenyl Diphenyl Phosphate, Tricresyl Phosphate, Trixylenyl Phosphate, Mixed Triaryl Phosphate & Isopropyl Phenyl Diphenyl W/ Cover. [R] . 2000

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An intracellular phosphate buffer filters transient fluctuations in extracellular phosphate levels

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