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Notes: Expanded Potential for Recombinant Trisegmented Lymphocytic Choriomeningitis Viruses: Protein Production Antibody Production and In Vivo Assessment of Biological Function of Genes of Interest

机译：注意：重组三段性淋巴膜脑膜炎病毒的扩展潜力：蛋白质生产抗体生产和目的基因的生物学功能的体内评估。

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The recombinant engineering of trisegmented lymphocytic choriomeningitis virus (LCMV) to express two genes of interest was recently reported. We used this technology to efficiently express green fluorescent protein (GFP) and the immunoregulatory gene product interleukin-10 (IL-10) in vitro, assess IL-10 function in vivo during viral meningitis, and generate specific, robust monoclonal antibody responses to IL-10. Tripartite viruses were attenuated in wild-type and TLR7^−/− mice. However, IFNAR1^−/− mice sustained systemic viral replication when 2 nucleotide substitutions from a persistent LCMV variant were present. These findings demonstrate the utility of tripartite LCMV in vitro and in vivo to study genes in the context of a well-defined model system.

机译：最近报道了三段性淋巴膜脑膜炎病毒（LCMV）的重组工程，以表达两个目的基因。我们使用该技术在体外有效表达绿色荧光蛋白（GFP）和免疫调节基因产物白介素10（IL-10），评估病毒性脑膜炎在体内的IL-10功能，并产生针对IL的特异性，强健单克隆抗体反应-10。三方病毒在野生型和TLR7 ^{-/-小鼠中减毒。但是，当存在来自持久性LCMV变体的2个核苷酸取代时，IFNAR1 ^{-/-小鼠可维持全身病毒复制。这些发现证明了在确定的模型系统的背景下，三方LCMV在体外和体内用于研究基因的实用性。}}

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期刊名称 Journal of Virology
作者
Daniel L. Popkin; John R. Teijaro; Andrew M. Lee; Hanna Lewicki; Sebastien Emonet; Juan Carlos de la Torre; Michael Oldstone;
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年(卷),期 2011(85),15
年度 2011
页码 7928–7932
总页数 5
原文格式 PDF
正文语种
中图分类人体病毒学（致病病毒）;病毒（滤过性病毒）;
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专利

1. Generation Of Recombinant Lymphocytic Choriomeningitis Viruses With Trisegmented Genomes Stably Expressing Two Additional Genes Of Interest [J] . Sebastien F. Emonet, Lucile Garidou, Dorian B. McGavern, Proceedings of the National Academy of Sciences of the United States of America . 2009,第9期

机译：具有稳定表达两个额外目的基因的三段基因组的重组淋巴细胞绒毛膜脑膜炎病毒的产生。
2. Detection of the Antibody to Lymphocytic Choriomeningitis Virus in Sera of Laboratory Rodents Infected with Viruses of Laboratory and Newly Isolated Strains by ELISA Using Purified Recombinant Nucleoprotein [J] . Kazuhiro TAKIMOTO, Motoko TAHARAGUCHI, Shigeru MORIKAWA, Experimental Animals . 2008,第4期

机译：纯化重组核蛋白酶联免疫吸附测定（ELISA）法检测感染了实验室和新分离株的实验室啮齿动物血清中的淋巴细胞性脑膜炎病毒抗体
3. Production of Polyclonal Antibodies to the Recombinant Potato virus MTI Production of Polyclonal Antibodies to the Recombinant Potato virus M (PVM) Non-structural Triple Gene Block Protein 1 and Coat Protein [J] . Cerovska N, Moravec T, Plchova H, Journal of Phytopathology . 2012,第5期

机译：重组马铃薯病毒MTI多克隆抗体的生产重组马铃薯病毒M（PVM）非结构性三联基因阻断蛋白1和外壳蛋白的多克隆抗体的生产
4. RNA Silencing Suppressors Boost the Production of Recombinant Proteins and Viruses [C] . Peter de Haan, Alfred Engel, Walter de Vries, European Society of Animal Cell Technology . 2010

机译：RNA沉默抑制剂促进了重组蛋白和病毒的生产
5. The infection kinetics of baculovirus: Mathematical models of the trafficking of acid-dependent enveloped viruses, enhancement of recombinant protein production, and design of infection regimens. [D] . Dee, Kennie Uy. 1996

机译：杆状病毒的感染动力学：酸依赖性包膜病毒的运输，增强重组蛋白生产以及感染方案设计的数学模型。
6. Generation of recombinant lymphocytic choriomeningitis viruses with trisegmented genomes stably expressing two additional genes of interest [O] . Sebastien F. Emonet, Lucile Garidou, Dorian B. McGavern, 2009

机译：具有三段基因组的重组淋巴细胞脉络膜脑膜炎病毒的产生该基因组稳定表达另外两个目标基因
7. Expanded Potential for Recombinant Trisegmented Lymphocytic Choriomeningitis Viruses: Protein Production, Antibody Production, and In Vivo Assessment of Biological Function of Genes of Interest ▿ [O] . Popkin, Daniel L., Teijaro, John R., Lee, Andrew M., 2011

机译：重组三段性淋巴膜脑膜炎病毒的扩展潜力：蛋白质生产，抗体生产以及相关基因生物学功能的体内评估

Notes: Expanded Potential for Recombinant Trisegmented Lymphocytic Choriomeningitis Viruses: Protein Production Antibody Production and In Vivo Assessment of Biological Function of Genes of Interest

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