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首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >Vesicle association and exocytosis at ribbon and extraribbon sites in retinal bipolar cell presynaptic terminals
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Vesicle association and exocytosis at ribbon and extraribbon sites in retinal bipolar cell presynaptic terminals

机译：视网膜双极细胞突触前末端的丝带和丝带外部位的囊泡缔合和胞吐作用

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Synaptic vesicles release neurotransmitter by following a process of vesicle docking and exocytosis. Although these steps are well established, it has been difficult to observe and measure these rates directly in living synapses. Here, by combining the direct imaging of single synaptic vesicles and synaptic ribbons, I measure the properties of vesicle docking and evoked and spontaneous release from ribbon and extraribbon locations in a ribbon-type synaptic terminal, the goldfish retinal bipolar cell. In the absence of a stimulus, captured vesicles near ribbons associate tightly and only rarely undock or undergo spontaneous exocytosis. By contrast, vesicle capture at outlier sites is less stable and spontaneous exocytosis occurs at a higher rate. In response to a stimulus, exocytic events cluster near ribbons, but show no evidence of clustering away from ribbon sites. Together, the results here indicate that, although vesicles can associate and fuse both near and away from synaptic sites, vesicles at synaptic ribbons associate more stably and fusion is more tightly linked to stimuli.
机译：突触小泡通过跟随小泡对接和胞吐过程释放神经递质。尽管已经很好地确定了这些步骤，但是很难直接在活突触中观察和测量这些比率。在这里，通过结合单个突触小泡和突触带的直接成像，我测量了小泡停靠的特性，以及在带状突触末端金鱼视网膜双极细胞中从带和色带外诱发和自发释放的特性。在没有刺激的情况下，被捕获的带状囊泡紧密结合在一起，很少脱离或自发的胞吐作用。相比之下，离群位点的囊泡捕获不稳定，自发胞吐发生率更高。作为对刺激的响应，胞外事件聚集在丝带附近，但是没有证据表明聚集离开丝带部位。在一起，这里的结果表明，尽管囊泡可以在突触部位附近和远离突触部位结合和融合，但突触带上的囊泡更稳定地缔合并且融合与刺激更紧密地联系在一起。

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