• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Proceedings of the National Academy of Sciences of the United States of America >One low-dose exposure of gold nanoparticles induces long-term changes in human cells
【2h】

One low-dose exposure of gold nanoparticles induces long-term changes in human cells

机译：低剂量接触金纳米颗粒会诱导人体细胞发生长期变化

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We report the in vitro long-term (20 wk) changes in cells exposed to well-characterized gold nanoparticles (Au NPs) with varying shapes and surface coatings under both chronic (exposure to Au NPs continuously over 20 wk) and nonchronic (initial acute cell exposure to Au NPs, followed by 20 wk in NP-free cell media) conditions. Both chronic and nonchronic Au NPs exposures at low dose induce modifications at the gene level after long periods. In attempt to overcome from the injuries caused by nanoparticle exposure, genes related to oxidative stress, cell cycle regulation, and inflammation are among those presenting differential expression levels. Surprisingly, the nonchronic exposure induced more gene expression changes than its chronic counterpart and the stress effects caused by this type of exposure were sustained even after 20 wk without any additional NP exposure. NP surface chemistry played an important role in the alteration of gene regulation. Overall, our data suggest that (i) cells can adaptively respond to chronic, low-level NP insults; (ii) the cell stress response is not reversible over time upon removal of NPs upon acute, nonchronic exposure; and (iii) polyethylene glycol is not as benign a surface chemistry as is generally supposed.
机译：我们报告了长期（20 wk）暴露于特征丰富的金纳米颗粒（Au NPs）的细胞的长期（20 wk）变化，该变化在慢性（长期暴露于Au NPs超过20 wk）和非慢性（初始急性）下均发生变化。细胞暴露于Au NPs，然后在无NP细胞培养基中暴露20周。低剂量的长期和非长期Au NPs暴露均会在长期后诱导基因水平的修饰。为了克服由纳米粒子暴露引起的伤害，与氧化应激，细胞周期调控和炎症相关的基因属于表现出差异表达水平的基因。出人意料的是，非慢性暴露比其慢性对应引起的基因表达变化更多，并且即使在20周后没有任何额外的NP暴露，这种类型的暴露引起的应激效应也得以维持。 NP表面化学在基因调控的改变中起着重要作用。总的来说，我们的数据表明：（i）细胞可以对慢性低水平的NP损伤做出适应性反应； （ii）急性，非慢性暴露后，去除NPs后，细胞应激反应无法随时间逆转； （iii）聚乙二醇的表面化学性质不如通常所认为的那么好。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号