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首页> 美国卫生研究院文献>The Scientific World Journal >Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers
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Genetic Diversity and Variability in Endangered Pantesco and Two Other Sicilian Donkey Breeds Assessed by Microsatellite Markers

机译：通过微卫星标记评估濒危的Pantesco和其他两个西西里驴品种的遗传多样性和变异性

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摘要

The genetic variability of Pantesco and other two Sicilian autochthonous donkey breeds (Ragusano and Grigio Siciliano) was assessed using a set of 14 microsatellites. The main goals were to describe the current differentiation among the breeds and to provide genetic information useful to safeguard the Pantesco breed as well as to manage Ragusano and Grigio Siciliano. In the whole sample, that included 108 donkeys representative of the three populations, a total of 85 alleles were detected. The mean number of alleles was lower in Pantesco (3.7), than in Grigio Siciliano and Ragusano (4.4 and 5.9, resp.). The three breeds showed a quite low level of gene diversity (He) ranging from 0.471 in Pantesco to 0.589 in Grigio. The overall genetic differentiation index (Fst) was quite high; more than 10% of the diversity was found among breeds. Reynolds' (D R) genetic distances, correspondence, and population structure analysis reproduced the same picture, revealing that, (a) Pantesco breed is the most differentiated in the context of the Sicilian indigenous breeds, (b) within Ragusano breed, two well-defined subgroups were observed. This information is worth of further investigation in order to provide suitable data for conservation strategies.
机译：使用一组14个微卫星评估了Pantesco和其他两个西西里土生驴品种（Ragusano和Grigio Siciliano）的遗传变异性。主要目标是描述当前品种之间的分化，并提供有用的遗传信息，以保护Pantesco品种以及管理Ragusano和Grigio Siciliano。在整个样本中，包括代表这三个种群的108只驴，共检测到85个等位基因。 Pantesco的平均等位基因数（3.7）低于Grigio Siciliano和Ragusano（分别为4.4和5.9）。这三个品种的基因多样性（He）水平较低，范围从Pantesco的0.471到Grigio的0.589。总体遗传分化指数（Fst）很高。在品种之间发现了超过10％的多样性。雷诺（DR）的遗传距离，对应关系和种群结构分析重现了同一张图片，揭示了：（a）Pantesco品种在西西里土著品种的背景下差异最大，（b）在Ragusano品种中，两个很好-观察确定的亚组。该信息值得进一步研究，以便为保护策略提供合适的数据。

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